Distribution of Soil Nematodes associated with Grapevine plant in Central Horticultural Centre (CHC), Kirtipur, Kathmandu
Date
2019
Authors
Journal Title
Journal ISSN
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Publisher
Central Department of Zoology
Abstract
Present study was carried out to find out the distribution of soil nematodes in the
rhizosphere of grape vine (Vitis venifera) in Central Horticultural Centre, Kirtipur. Soil
nematodes were extracted from 100 soil samples collected from rhizosphere soil of grape
vine i.e. 50 samples each during summer and winter season. Nematodes were extracted by
Cobb‟s (1918) seiving and decantation method and followed by Baermann‟s (1917)
funnel method. The further processing of nematodes was done by Seinhorst‟s (1959)
method in the laboratory of Central Department of Zoology, Tribhuvan University,
Kirtipur. Altogether seven species of nematodes were isolated from grape vine
rhizosphere soil samples; Tylenchorhynchus sp., Xiphinema sp., Longidorus sp.,
Mononchus sp., Diplogastrine sp., Rhabditis sp. and Mesorhabditis sp. . Among the
isolated species, Tylenchorhynchus sp., Xiphinema sp., and Longidorus sp. were parasitic
nematodes. Longidorus sp. were high in number in summer while Tylenchorhynchus sp.
showed high infestation during winter season. Beside plant parasitic nematodes, predator
nematodes were also isolated from the same soil samples. Mononchus sp. was found
high in summer season however Diplogastrine showed no difference in both summer
and winter seasons. Similarly several other free living nematodes were also isolated.
Rhabditis sp. and Mesorhabditis sp. were free living nematodes equally distributed in
both summer and winter seasons. In conclusion, the grape vine of Central Horticultural
Centre were infected with plant parasitic nematode throughout the year. In order to
increase the plant productivity treatment for plant parasitic nematode is necessary and
recommended for the same.
Description
Keywords
Distribution, Study