Antioxidant, Antimicrobial, Cell Cytoxicity assay and Phytochemical Screening of Some Medicinal Plants

Abstract

The plant parts are used from very prehistoric period of time. All living beings are completely dependent on the plant as food to cure different types of problem they faced. Similarly Rosa spp. that are always around us used as decorative, fencing, aromatic, ornamental to sign of love because of its importance on the physiological and physical effects on the human and organisms. Methanol extracts of the compound was used because large number of plant are screened under the methanol so that, methanol as the solvent of the plant extraction. Tinospora cordifolia was also used for observing the comparative study as it has been widely used for medicinal effects. Maximum effects was observed in the Rosa spp (purple) was hybrid plant that has 217.19 ± 4.49. Similarly, flavonoids contents in them Rosa brunonii has 161.75± 14.22.Other spp. also have similar types of readings. So that the study of radical scavenging activity was carried out where the IC50 value was calculated in the mean weight and SD so that all plant extracts has similar range value was obtained which shows the similar type of the radical scavenging activity by all the plant extracts. Rosa spp (white) has effects of 60.95 ± 3.42 for Tinospora cordifolia has 52.54 ± 0.73. Antimicrobial effects are also shown by the all plant extract in 50 to 100µl/gm which was observed in zone of inhibition by paper disc methods using both positive and negative control. The VERO cells with passage no 28 was used for observing cell cytoxicity effect that was also done by MTT assay and reading was taken spectrophotometrically so that the effect was seen by calculating % inhibition which gives the value of IC50 value that helps to find the inhibiting value for which Tinospora cordifolia has 128.47 ± 44.42 for Rosa spp (white) 110.76 ± 21.54.Those species required high concentration of extracts compare to Rosa spp and Rosa brunoii.GC-MS analysis was carried out to know the types of chemical compounds that are present in plant extracts. Those compounds are responsible for the various effects. The results shows the importance of ethanomedicinal plants that are responsible for curing various disease. The bioactive compounds are responsible for the therapeutic properties. Different types of analysis and characterisation of each compounds can leads to upgrade the major findings. Key words: Rosa species, Tinospora cordifolia, antioxidant, antimicrobial, cell cytotoxicity,GCMS, phenol, flavonoid.