Micropropagation of Vanda Tessellata (Roxb.) Hook.Ex.G.Don

Abstract

Vanda tessellata (Roxb.)Hook.ex G. Don is a monopodial orchid and epiphytic in habitat with leafy stem of medium size plant body. It is renowned for its horticultural and high medicinal value as well. The aim of its study was to develop an efficient protocol for micropropagation of Vanda tessellata Roxb. In the present study the protocorms were first tried in full MS, half MS and quarter MS medium without addition of any plant hormones and other organic additives. Quarter strength of MS medium was found to be effective medium for the earlier development of shoots and shootbuds from protocorm. Therefore further experiment was carried out in this medium. Shoot multiplication was carried out in different concentrations and combinations of GA 3 , CW, BAP, BAP plus 6% sucrose and NAA. Among them, quarter strength MS medium supplemented with 0.5 mgl -1 BAP was found to be effective for shoot multiplication, MS medium supplemented with 1.5 mgl -1 BAP plus 6% sucrose was found to be effective for shoot bud development and quarter strength of MS medium supplemented with 0.5 mgl -1 NAA favored the root formation. Higher number of leaves was found in quarter strength of MS medium supplemented 1.5mgl -1 GA 3 and longest leaf length was found in quarter strength MS medium supplementedwith 0.5 mgl -1 GA 3 .This present study reveals that this species favors BAP for shoot multiplication as compared to other hormones and organic additives used. This protocol might be useful for in vitro shoot bud formation and multiplication from protocorms for mass propagation and ex situ conservation of this valuable plant that is used as medicinal and horticultural aspects.