Tiwari, Vijay2022-12-182022-12-182014https://hdl.handle.net/20.500.14540/13881Antibiotic resistance is now a global concern. The emergence and spread of antimicrobial resistance due to the production of β-lactamases represent a clinical threat because of their broad spectrum of activity. The present study was conducted at Alka Hospital from Ocotober 2011 to December 2012 with the objectives to study the emergence of extended spectrum β-lactamase production in common bacterial isolates from urine sample. During this period, 1699 mid-stream urine samples collected were investigated to determine UTI by conventional culture technique and microscopy. Only 16% (n=271) out of 1699 samples showed significant bacterial growth. The maximum number of growth was observed in age group 20-30 yrs (27.3%), followed by age group 30-40 (20.30%). The high culture positivity was seen among female 17.9% (n=193) than in males 12.4% (n=78). Altogether nine different species of bacteria were isolated among which Escherichia coli 67.50% (n=183) were found the most predominant organisms followed by Staphylococcus aureus 10.70% (n=29), Klebsiella pneumoniae 7% (n=19). Multidrug resistance (MDR) was observed in 70.11% (n=190) of total bacterial isolates. Seventy seven (28.4%) isolates were confirmed for ESBL production. ESBL production was found highest in E.coli 31.6% (n=183) followed by K. pneumoniae 31.50% (n=19), Pseudomonas aeruginosa 27.30% (n=11), Acinetobacter spp. 26.70% (n=15).en-USBacteriuriaUrinary tract infectionMid-stream urineMultidrug-resistanceThesis