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Browsing Thesis & Dissertations by TU Institute "Central Department of Biotechnology"
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Item A study of NAK cheese functional aspects(2015) Katawal, Ishwar; Ganga P. KharelAvailable in fulltextItem Accuracy of Lymphocyte counts from Unicel DxH 800 in ß-Thalassemia/HHE patients having various numbers of Nucleated red blood cells(2019) Timilsena, Sunil; Krishna Das ManandharAvailable in fulltextItem Ageing of bone marrow derived mesenchyman stem cells in relation to nutrient starvation(2015) Poudel, Aarati; Tilak R. ShresthaAvailable in fulltextItem Analysing the synergistic effect of Lytic bacteriophage and antibiotics against multidrug resistant Klebsiella pneumoniae causing urinary tract infection(2024) Dahal, Puja; Pragati PradhanAvailable in fulltextItem Analysis of degradable household kitchen waste and harnessing bioelectricity from it by enhancing microbial fuel cell (MFC) performance(2023) Paudel, Pravesh; Jarina JoshiAvailable in full textItem Analysis of phytochemical and bioactivity of underutilized Nepalese plants(2016) Kayastha, Sandipty; Bal Hari PoudelAvailable in fulltextItem Antibacterial activity of loctobacillus casel against staphylococcus aureus and isolation and purification of bacteriocin from lactobacillus(2014) Bhattarai, Susmita; Rajani MallaAvailable in fulltextItem Antioxidant, antibacterial and cell toxicity assay of some local herbal plants(2014) Maharjan, Prashanna; Ram Chandra BasnyatAvailable in fulltextItem Antioxidant, antimicrobial, cell cytoxicity assay and phytochemical screening of some medcinal plants(2017) Thapa, Dipesh; Krishna Das ManandharAvailable in full textItem Application of Bacteriophage in Nosocomial infection control, A new concept to disinfect Hospital environment(Department of Biotechnology, 2021) Upreti, HimaniIntroduction: Health care associated infections (HAIs) are one of the important public health problems which might result in significant rise in mortality and morbidity, predominantly in immune compromised patients of Intensive Care Unit (ICU). A study showed that in the Asian region, risks of HAIs have been estimated to be 2-20 times higher than in developed countries up to 25% of hospitalized patients having acquired infection (Ling et al., 2015) and also a report shows that in United States, roughly 9.2 out of every 100 patients acquire a nosocomial infection, according to Healthline and among them also some HAIs is quite serious and potentially life-threatening. The common bacteria causing nosocomial infections include Pseudomonas aeruginosa, klebsiella pneumonia, Acinetobacter baumanii, Enterococci etc. (Khan, Baig, & Mehboob, 2017). Therefore, disinfectants like alcohols, hypochlorite, hydrogen peroxide, amyl phenol, glutaraldehyde etc. are widely used in hospital to kill these organisms which cause NAs but sometimes these chemical disinfectants might be hazardous, irritants and toxic to us and environment. Among many alternatives, bacteriophage mediated bio-control of the pathogenic bacteria is considering as one of the best options. Our objective of the study is to isolate, identify the pathogenic bacteria from the hospital environment and evaluate the efficacy of newly isolated lytic bacteriophage to minimize the bacterial load on hospital fomites. Methodology: The study time period of this research was 6 months and, in this study, we isolated phage against Pseudomonas aeruginosa bacteria collected from ICU of Teku hospital. Then most potent phage was characterized morphologically and Physiochemically. Burst size was obtained from one step growth curve. Intraspecific and interspecific host range was assessed by spot assay. During this research work, we used sterile fomite cloth pieces and marble tile as they are most potent sources were bacteria reside. Firstly, we contaminate the fomites and tiles with Pseudomonas aeruginosa bacteria then after we used Pseudomonas aeruginosa phage to decontaminate those cloths and tile. Result: Altogether 16 bacterial strain, 4 Pseudomonas aeruginosa and 12 Staphylococcus aureus were confirmed by Gram staining and Biochemical test and among them 2 Pseudomonas aeruginosa strains and 5 Staphylococcus aureus strains were found to be Carbapenem resistance and Multi Drug Resistant (MDR) respectively. Total six bacteriophages against Pseudomonas aeruginosa were isolated from different sewage samples. And one of the most potent phage P4 was characterized morphologically and physiochemically. Burst size of the phage was found to be 28 virions per bacterium. Protein profiling was done by SDS-PAGE where protein band between 20-250Kda were found and Phage P4 belongs to order Caudovirales and family Siphoviridae. Similarly, Phage was found to tolerate temperature of 70°C for 20 minutes, pH 3-12, exhibiting multiple host range as well. Decontamination assay was done on the sterile fabric cloth which showed that the P4 phage having MOI value 1 showed higher rate of decontamination with log reduction of 1 and p-value (0.002) i.e., significant. Further Comparison was done between P4 phage and normal disinfectant Phenol where we found that single phage has more disinfectant rate than phenol. xi Similarly, time period up to which bacteriophage can show their effectivity as disinfectant was also done and it showed highest level of effectivity up to 6 hours at MOI 1 with the CFU/ml log reduction of 1. Conclusion: The result from the present thesis reveals several characteristics of the bacteriophage (P4), for instance, effective lytic capability, multiple host range, and stability in wide range of pH and temperature. Higher rate of decontamination with the log reduction of 1 CFU/ml and p-value (0.002) was showed by P4 phage having MOI value of 1. While doing comparison single phage have more disinfectant rate than normal disinfectant & P4 phage can show its highest level of effectivity up to 6 hrs at MOI 1. Keywords: Nosocomial Infection, disinfectant, Pseudomonas aeruginosa, bacteriophage, decontaminationItem Assessment of genetic diversity, and barcoding and chemical analysis of neopicrorhiza scrophulariifiora (PENNELL) hong of Nepal(2014) Gyanwali, Gaurav Chandra; Jyoti MaharjanAvailable in fulltextItem Bioactive compounds and biological activities of some medicinal plants from Central Nepal(2016) Ghimire, Gyandra Prasad; Krishna Das ManandharAvailable in fulltextItem Biochemical and molecular study of cellulase from bacillus lichniformis isolated from hot spring water Gorkha(2013) Giri, Gorkha Raj; Jyoti MaharjanAvailable in fulltextItem Bioinformatic approach for generation of a fusion protein of T-Snare Snap-23 containing a transmembrane Domain(2012) Sah, Govinda Prasad; Rajani MallaAvailable in full textItem Bioprospecting of some medicinal plants of Nepal based on Ethnobotanical knowledge of the Tamang community(2018) Mainali, Pratikshya; Krishna Das ManandharAvailable in full textItem Characterization of bacteriophges against multi- drug resistant pseudomonas aerugainosa analysis of multi host range bacteriophages(2017) Maharjan, Archana; Rajani MallaAvailable in fulltextItem Characterization of clinically important mutants of human secretagogain(2017) Thapa, Rashmi; Tilak R. ShresthaAvailable in fulltextItem Charaterization of myxobacteria by using molecular tools and identification of gene responsible for anti- microbial activity(2019) Subedi, Surendra Kumar; Rajani MallaAvailable in fulltextItem Charaterization, whole genome analysis and application bacteriophages to control biofilm produced by charbapenem resistant bacteria prioritized by world health organization(2018) Parajuli, Apshara; Rajani MallaAvailable in fulltextItem Cloning of ddi (DNA damage inducible) gene in pfcenv 3\ v4 transection vectors and production of recombinant generation of antibodies(2019) Majhi, Bhagawati; Krishna Das ManandharAvailable in fulltext