Distribution of Soil Nematodes associated with Grapevine plant in Central Horticultural Centre (CHC), Kirtipur, Kathmandu

Abstract

Present study was carried out to find out the distribution of soil nematodes in the rhizosphere of grape vine (Vitis venifera) in Central Horticultural Centre, Kirtipur. Soil nematodes were extracted from 100 soil samples collected from rhizosphere soil of grape vine i.e. 50 samples each during summer and winter season. Nematodes were extracted by Cobb‟s (1918) seiving and decantation method and followed by Baermann‟s (1917) funnel method. The further processing of nematodes was done by Seinhorst‟s (1959) method in the laboratory of Central Department of Zoology, Tribhuvan University, Kirtipur. Altogether seven species of nematodes were isolated from grape vine rhizosphere soil samples; Tylenchorhynchus sp., Xiphinema sp., Longidorus sp., Mononchus sp., Diplogastrine sp., Rhabditis sp. and Mesorhabditis sp. . Among the isolated species, Tylenchorhynchus sp., Xiphinema sp., and Longidorus sp. were parasitic nematodes. Longidorus sp. were high in number in summer while Tylenchorhynchus sp. showed high infestation during winter season. Beside plant parasitic nematodes, predator nematodes were also isolated from the same soil samples. Mononchus sp. was found high in summer season however Diplogastrine showed no difference in both summer and winter seasons. Similarly several other free living nematodes were also isolated. Rhabditis sp. and Mesorhabditis sp. were free living nematodes equally distributed in both summer and winter seasons. In conclusion, the grape vine of Central Horticultural Centre were infected with plant parasitic nematode throughout the year. In order to increase the plant productivity treatment for plant parasitic nematode is necessary and recommended for the same.