Study of A-Amylase Enzyme in hibition Activity and Antimicrobial Activity of Local Tea Camellia Sinensis (L.) Kuntze, Leaves of Buddleja Asiatica Lour, And Roots of Polygala Arillata Buch.-Ham. Ex D. Don

Abstract

A phytochemical and natural product rich medicinal plant is the alternative source for the discovery of new anti-diabetic agent. Diabetes mellitus (DM) is a fatal disease increasing prevalently and their complication then remedial drug with a large number of side effects. Therefore, to search the medicinal plants having the potential to inhibit the α-amylase key enzyme causing the diabetes mellitus is the main goal of the study. In this study, local tea C. sinensis, leaves of B. asiatica, roots of P. arillata, were subjected to phytochemical screening, and quantitative analysis of TPC was found to be 157.17 ± 18.79, 116.47 ± 6.62, and 44.27 ± 2.97 mg GAE/gm in dry weight of the extract. Similarly, TFC was found to be 37.07 ± 2.24, 34.28 ± 2.63 and 5.99 ± 1.00 mg QE/gm dry weight of the extract. Then, antioxidant DPPH assay was performed, IC50 values of methanolic extracts of B. asiatica, C. sinensis, and Quercetin were found to be 26.86±2.00, 19.15±4.32 and 2.36±0.13 μg/mL respectively.

In the case of α-amylase inhibition assay by starch-iodine method, IC50 values of methanolic extract of local tea C. sinensis, B. asiatica, and Acarbose standard were found to be 1737.65 ± 2.21, 1780.00 ± 3.06 and 55.74 ± 2.85 µg/mL respectively. On further analysis of α-amylase inhibition assay by DNS method in the fractions of local tea C. sinensis, and B. asiatica extracts IC50 values were found to be TH-1296.13 ± 94.46, TD675.97 ± 66.18,

TE-338.52 ± 3.78 μg/mL and KH-767.46 ± 18.44, KD-411.50 ± 79.07 and KE- 1051.62 ±12.05 μg/mL respectively in Hexane, dichloromethane, and Ethyl acetate fractions. Antimicrobial activity was performed and their zone of inhibition (ZOI) in local tea C. sinensis, leaves of B. asiatica, were found to be 17 mm and 12.5 mm against the gram-positive bacteria S. aureus. Finally, FT-IR analysis was also performed in ethyl acetate fraction of 20 % methanol in ethyl acetate and 100 % methanol column fractions of C. sinensis.

KEYWORD:- Phytochemical screening, 96-well plate microreaders, DPPH assay, α-amylase inhibition assay, Antimicrobial activity, FT-IR spectra.