Studies on Antioxidant and Xanthine Oxidase Inhibitory Activities of Coccinia Grandis (L.) Voigt, Datura Metel (Linn.) and Tagetes Patula (L.) from Rupandehi District of Nepal

Abstract

Nepal is great country having all the types of biodiversity which is suitable for the growth of different type plants. Different types of natural products and their biological activities can be obtained from these plants which could have great benefits in cure of different diseases. Phytochemical screening of different plant extracts showed the rich resources of secondary metabolites such as alkaloids, flavonoids, phenols etc. Calculations of TPC and TFC value of plant extracts showed that T. patula content highest value TPC (630.839±2.633 mgGAE/g) and TFC (79.605±1.808 mgQE/g) respectively. Antioxidant activities of these plant extracts studied by both DPPH and FRAP both showed D. metel with IC50

value of 0.039 mg/mL and 357.0365±0.682317 mm Fe (II)/L respectively, as a potent antioxidant among the selected three plant extracts. The antibacterial activity was studied by well diffusion method which showed plant extract of T. patula only showed the activity of 10 mm ZOI value at concentration of 50 mg/mL as compared to standard drug against S. aureus while other plant extracts did not showed any activity against both gram positive and gram negative bacteria. Xanthine oxidase (XO), an enzyme widely distributed among mammalian tissues, is associated with the oxidation of xanthine and hypoxanthine to form uric acid. This is the serious effect on the human being many people are suffering from this disease. The antigout activity of these plant extracts were studied by xanthine oxidase inhibition assay where methanolic extract of C. grandis with IC50 value 0.491 mg/mL showed the highest xanthine oxidase inhibition activity among selected plants, refers it as potent antigout. Since C. grandis was selected for column and FTIR analysis of different fraction was observed. FTIR analysis showed different carbonyl, phenolic, alkane compounds are present in the extracts. This study may provide scientific basis for the use of selected plants and may provide valuable information for further research.