Pattern of bacterial Isolates and antibiogram from open wound Infection Among The Indoor Patients of Bir Hospital

Abstract

The present study was conducted for a period of 9 months with an aim to identify the etiological agents causing wound infection along with their antibiotic susceptibility pattern among inpatients in Bir Hospital. In this study a total of 305 pus samples which consisted251(82.29%) pus swabs and 54 (17.70%) pus aspiration from the infected wounds were collected and analyzed. The etiological agents were isolated,identified by culture and biochemical tests and their susceptibility pattern to commonly used antibiotics were determined using standard protocols.The male patients were high in number(n=185)than females(n=120)and majority of patients belonged to age group 21-30 (n=76). Out of total 305pus samples, 197 (64.45%)samples showed growth; of which 135 (68.52%) samples showed single isolates and 62(31.47%) showed multiple isolates. The growth was found to be highest in burn ward(90.90%) followed by post operative ward (80%) and lowest was in plastic surgery ward(33.33%).Atotalof253 bacterial isolates were found which belonged to 15 different species;of which155 (61.2%)were Gram negative and 98 (38.8%)were Gram positive bacteria.Among Gram positive bacteria, S.aureus(83.67%) was most common followed by CONS(7.14%), non haemolytic streptococci (4.08%),unidentified Gram positive rods (3.06%) and haemolytic streptococci (2.04%).Among Gram negative bacteria,E. coli (38.7%) was most common followed by P. aeruginosa (25.16%),Acinetobacter spp.(10.96%),K.oxytoca(7.74%),P. mirabilis(5.16%),K. pnemoniae(4.51%),P. vulgaris(2.58%),Enterobacter spp.(2.58%),C. freundii (1.93%)and Providencia spp.(0.64%).Direct smear Gram staining and culture were found to be correlated (r = 0.99).Among the antibiotics used, the most effective antibiotic for overall bacterial isolates was found to be Ciprofloxac in with asensitivity of 67.58%. For Gram positive isolates, the most sensitive antibiotic was Of loxacin(70.40%)while among the Gram negative isolates, Ciprofloxacin (69.03%)was the mostsensitive antibiotic.Hence,etiological agents of wound in fection along with their antibiotic susceptibility pattern were determined. xy Key words:wound infection, indoor patients,bacterial isolates, antibiogram