Browsing by Subject "P. aeruginosa"
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Item Bacterial Isolates from Ear Discharge of Patients suffering from Otitis Media and Their Antibiotic Susceptibility Pattern(Department of Microbiology, 2012) Suwal, RamitaThis study was conducted to identify the aerobic bacterial pathogen causing otitismedia and determine their antibiotic susceptibility pattern. Aural discharge samples were collected from the patients visiting the Ear Nose ThroatOut Patient Department of Shree Birendra Hospital, Chhauni, who were diagnosed with chronic suppurative otitis media and acuteotitis media. Standard microbiological procedures were employed to isolate and identify the causative agents of the infection and determine the antibiotic susceptibility pattern of isolated pathogen. Among total 153 samples,128(84.31%) samples were positive.119(77.7%)samples produced single organism and9(5.8%) samples gave mixed growth.The total of 136 isolates was obtained. Gram positive organisms (64%) were predominant. The most common isolates were Staphylococcus aureus from70(51.5%) cases followed by Pseudomonas aeruginosa from 27 (19.9%) cases.Other organisms isolated were Coagulase negative staphylococci (11.8%), Klebsiella species (6.6%),Escherichia coli (5.1%), Citrobacter fruendii (2.9%),Proteus mirabilis (0.7%), Streptococcus pyogenes (0.7%) and Haemophilus influenzae(0.7%).S. aureus showed greater sensitivity(67.1%) to cefoxitin than other antibiotics tested. Antibiotic susceptibility test of P. aeruginosa showed100%sensitivity to imipenem, 92.6% to piperacillin, 88.9% each to ceftazidime and amikacin and 74.1% to ciprofloxacin. Similarly, most E. coliisolates (71.4%)were sensitive to gentamicin, C. fruendii (100%) to gentamicin, Klebsiella sps(77.8%) to ciprofloxacin and gentamicin each.S. aureuswas found to be the most common etiological agent of otitis media followed byP.aeruginosa. Keywords:Otitis media, Chronic suppurative otitis media,S. aureus,P.aeruginosaItem Characterization of Pseudomonas Aeruginosa Isolated from Intensive Care Units in Shahid Gangalal National Heart Center(Department of Microbiology, 2011) Bhandari, ShivaPseudomonas aeruginosa is a gram negative bacterium responsible for several nosocomial infections in highly immunocompromised and catheterized patients in Intensive Care Unit (ICU). In order to characterize and determine the prevalence of P. aeruginosa in the ICUs of Shahid Gangalal National Heart Center, a six month cross sectional microbial study was undertaken. A total of 700 clinical specimens and 360 surface swab samples from ICU were collected and analyzed for bacteriological profile. The bacterial isolates were identified by biochemical testing. Antibiotic susceptibility testing of isolated bacteria was performed by Kirby Bauer disc diffusion technique. In all clinical samples analyzed, P. aeruginosa was detected in 66 (9.43%) samples while in all surface swab samples analyzed, P. aeruginosa was detected in 60 (16.67%) samples. 48 (72.7%) of clinical samples yielded mucoid strains while it was only 24 (40%) for surface swab samples. Among clinical samples, 61 (92.4%) were pigment producing strains while 5 (7.6%) were non-pigmented strains. Likewise, among the surface swab samples, 45 (75.0%) were pigment producing strains of P. aeruginosa while 15 (25.0%) were non-pigmented strains. Antibiotic Susceptibility Test demonstrated that among clinical isolates 56 (84.8%) were sensitive to cefoparazone-sulbactam followed by 42 (63.6%) to polymixin-B and 36 (54.5%) to piperacillin-tazobactam, while among surface swab sample isolates more than 90% isolates were sensitive to most of the common antibiotics used. 59 (89.4%) Multi-drug Resistant P. aeruginosa (MDRPA) were isolated from clinical samples while it was only 7 (11.7%) from surface swab samples. This study signified that P. aeruginosa was an important cause of infection in patients admitted in the ICUs and it could be present in the inanimate surfaces of ICUs posing threat to the ICU patients. Regular monitoring of antimicrobial susceptibility and rational use of antibiotics would be the essential steps to eliminate possible outbreaks of MDRPA in the ICUs.Item Screening of Novel Genes Involved in Bio-Film Mediated Resistance to Antibiotics in MDR Pseudomonas aeruginosa(Department of Biotechnology, 2022) Manandhar, Padma RatnaAvailable with full text