Browsing by Subject "Pulmonary Tuberculosis"
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Item Isolation and Identification of the Etiological Agent of Pulmonary Tuberculosis in Patients Visiting National Tuberculosis Center, Thimi, Bhaktpur(Department of Microbiology, 2006) Singh, SudeepDuring the study period of June 2005 to May 2006 a total of 200clinicallysuspectedsputum samples were examined by ZN staining and cultured in Ogawa medium.Primaryculture samples were subcultured in the LJ medium. The subcultures wereobserved for their cultural characters for 4 weeks and then subjected for biochemicaltests for their confirmation asM.tuberculosis.From the 200 subculturesNiacin,Nitratereduction, 68ºC labile catalase test and growth on PNB containing medium wasperformed. Out 200 samples 190 (95%), 189 (94.5%), 6 (3%) and 7 (3.5%) werepositive for Niacin, Nitrate reduction, 68ºC labile catalase and growth on PNBcontaining medium and 10 (5%), 11 (5.5), 194 (97%) and 193 (96.5%) were negativefor the respective tests. Out of 200 samples 189 (94.5%) were positive for Niacin and Nitrate reduction andnegative for 68ºC labile catalase and growth on PNB containing medium whichconfirms that they wereM. tuberculosis. Thus with the combination of above mentioned tests 189 (94.5%) out of 200 culturepositive were confirmed asM. tuberculosisand the rest may be MOTT. Biochemicaltests although time consuming is a very good alternative to new quick methods likePCR and NAA.Item Pulmonary Tuberculosis: Prevalence and Awareness in Ward No.8 and 9 of Janakpur, Dhanusha(Department of Zoology, 2008) Tiwary, Pankaj KumarTuberculosis remains a major global public health problemparticularly in developing and underdeveloped countries. In case of Nepalalso, it is one of the major health problems. A study was conducted in 250peoples of ward 8 and 9 of Janakpur Municipality. Study work wascarried out in Janakpur Municipality of Dhanusha district during May andJune 2006. A total of 250 sputum samples were randomly collected bydirect home visit from suspected persons and the samples were examinedby Ziehl-Neelsen (Z-N) staining method. Out of 250 samples, a total of 9(3.6%) AFB positive cases were detected. Out of total AFB positive cases66.66% were males and 33.33% females but the sex wise difference inthe prevalence of tuberculosis was not found to be statistically significant.High incidence of disease prevailed among productive age group (20-40years) but difference with their age-group was not found to be statisticallysignificant. In the study area, 84% respondents knew about TB disease.Smoking is the predisposing factor of tuberculosis where 56%respondents were current smokers and smoked between 10 to 20cigarettes/day.