Morphogenetic Studies on Different Explants Ofrauvolfiaserpentina(L.) Benth. Ex. Kurzin-Vitroandacclimatization

Abstract

Morphogenetic studies on different explants ofRauvolfia serpentina(L.) Benth.ex Kurz in-vitroand in-vivo were carried out. Explants were taken from in-vitrogrownplants on MS media. Various vegetative parts like node, leaf, root and shoot-tip werecultured. The effect of growth hormones on morphogenesis was discussed. The explants cultured on the MS media with 2,4-D (0.5-3 mg/l) developed intocalli from cut part. The node, leaf and root started to develop calli from thecut part in the7,8 and 9 days of culture. The growth of calli was found better in the 0.5 mg/l 2, 4-D thanin 3 mg/l 2, 4-D and the leaf explants gave more vigorous callus in primary culture thannode and root explants. Further sub-culture of the root callus on the fresh media of 2, 4-Donly increased the mass which turned brownish in 8 weeks of sub-culture. The 2, 4-Dinduced callus onsub-culture on BAP and NAA of various concentration differentiatedroots only. The transfer of nodal callus on fresh media (MS+BAP+NAA in combination)differentiated shoots and roots. Nodes were also cultured on MS medium supplemented with nine differentcombinations and concentrations of BAP and NAA. Maximum number of shoots wasfound in the MS+2 mg/l BAP (average 16.001.10) and best shoot elongation was foundin the MS+1.5 mg1l BAP (average 7.42 0.62 cm) in the 8 weeks of culture. Similarly,the leaf explants were cultured on MS medium supplemented with nine differentconcentrations of BAP and NAA Response was observed only in combination and 0.5mg/l NAA singly. MS+ BAP (2 mg/l) + NAA (0.5 mg/l) gave plantlets from cut endwhile in other hormone concentrations green callus with red and white patches wasproduced which differentiated only roots. Shoot tips cultured in MS+Kn (0-3 mg/l) was not effective to produce multipleshoots as compared to BAP. Shoot tips at basal part produced callus which differentiatedshoots (1, 3 mg/l Kn) 6-week oldin-vitromultiplied shoot without rooting was taken forin-vivorooting.In-vivo rooting was done with or without treating the shoots with IAA (3 mg/l for 1hour). 100% shoots were rooted in IAA treated shoots in the 3 weeks of plantation while90% of shoots were rooted in the 4 weeks of plantation in shoots that were not treatedwith IAA.