Comparative Study on Micropropagation of some Citrus Spp. Innepal

Abstract

In this investigation,invitrogermination, multiplication andacclimatiza-tionof virus free citrus plants was done. Lime seeds were germinated after 5 weeks of culture on CEMsupplemented with 500 mg/l of GA The germination of lemon seeds was foundto be better than of lime. Lemon did not express ployembryony but its seedlingsgave multiple shoots which was not expressed by lime. 3. Better multiple shoots were obtained from the nodal explants of limeAO (6) on MS-medium supplemented with 0.5mg/l BAP + 0.5 mg/l NAA. Thebest multiple shoots of lime BO 1 (15) were obtained from the nodal explantswhen cultured in MS-medium supplemented with 0.5 mg/l BAP + 1 mg/l NAA.Under similar conditions (MS-medium, supplemented with 1mg/l BAP +0.1mg/l NAA) among lime AO 1 1 (6), lime BO (15), lemon and Troyer Citrange,the best multiplication was observed in Troyer Citrange. 1 Basically, the main purpose of meristem culture was for viruselimination. The meristems were selected from the young shoot apices andcultured in filter paper bridge on MS-medium supplemented with BAP1mg +NAA0.1mg/l. Four meristems (16.16%) could be saved and multiplied for 6months attaining the size of about 0.5 cm which eventually turned brown anddied. Rooting of lime AO (6) in IBA 1 mg/l was found to be better onMS-medium. Similarly, 0.5 mg/l of NAA was also found to be optimum.Acclimatization was carried on non-sterile sand and soil mixture (1:1). About37.71% single rooted plants and 46.15% multiple rooted plants weresuccessfully acclimatized. The acclimatization was done inside screen house. 1 The whole protocol was developed for lime, lemon, mandarin orange andtrifoliate orange under in vitro(controlled) condition. All initial explants wereCTV-free and finally rooted and then successfully acclimatized.Lime AO (6)was also found to be CTV free by DAS-ELISA test. 1