Microbiological Study on Gastroenteritis of children From Kanti Children's Hospital With Reference Tocyclospora and Rotavirus Infection

Abstract

A study was conducted from May to September 2006 among children below 5 years of age attending Kanti Children's Hospital who were suspected of gastroenteritis. Among five hundred samples, three hundred and eighty six were taken from patient suffering from diarrhoea and one hundred and fourteen were non-diarrhoeal. Samples were collected and investigated in Health Research Laboratory, Institute of medicine by wet saline, iodine mount, and Ziehl Neelson staining for Cyclosporaand other parasites, Enzyme Immuno Assay for rotavirus and selective culture method for bacteriological investigation. The prevalence of enteropathogens observed was (52.1%) in diarrhoeal cases and (39.5%) in non-diarrhoeal cases. Out of 386 diarrhoeal samples (16.8%) was positive for rotavirus, (7.8%) forCyclospora, (3.4%) for bacteria and (24.1%) for other parasites. Out of 114 non-diarrhoeal samples(4.8%) was positive for rotavirus, (6.14%) for Cyclospora, one case for bacteria and (28.1%) for other parasites. Among diarrhoeal case rotavirus infection was seen highest in the age group 7-24 months (21.6%),month of September (28%) and in males (17.95%) than in females (14.8%). Cyclosporiasis was foundhighest in the age group 0-6 months (12.5%)and in month of July (16.45%). Among non-diarrhoealcases, rotavirus infection was seen highest in the age group 7-24 months (5.7%), month of May(13.3%) and in males (4.54%) than in females (4.16%). Cyclosporiasis was found highest in the age group 25-60 months (12%),and in month of June (15.78%). Among the bacterial pathogens isolated,Vibriospp (2.6%) was the major causative agent followed by Salmonellaspp. (.12%) and Shigellaspp. was not found in a single case. Among diarrhoeal cases, the prevalence rate of bacterial pathogen was higher in the age group 25-60 months (2.3%), in the month of September (16%) and in females (1.8%). Among non-diarrhoeal cases, it was only found in the agegroup 25-60 months (0.9%). In non-diarrhoeal cases, it was found only in the month of August (3.3%)and only females were infected (0.9%). In diarrhoeal cases, the protozoans were Entamoeba histolytica(9.3%),Giardia lamblia(8.3%),Cyclospora(7.8%),Entamoeba coli (1.1%),Cryptosporidium parvum(0.8%),Blastocystis hominis(1.8%). Whereas in non-diarrhoeal cases,Giardia lamblia(10.5%),Entamoeba histolytica(7.0%),Cyclospora (6.1%), Entamoeba coli (0.8%), Cryptosporidium parvum(0.7%) and Blastocystishominis(3.5%). In diarrhoeal cases, the helminthes wereAscaris lumbricoides(1.1%),Hymenolepsis nana(1.1%),Trichuris trichuria(0.5%),Taeniaspp. (0%), whereas in non-diarrhoeal cases,Ascaris lumbricoides(1.2%)and Trichuris trichuria(1.2%),Hymenolepsis nana(0.9%),Taenia spp.(0.9%). The prevalence of enteropathogen was found highest in the children consuming tap water and children having nausea and vomiting.