Synthesis and Characterization of Hydroxyapatite from Caprine (Goat) Bone for Microbial Adsorption and Antimicrobial Study
Date
2021
Authors
Gautam, Bibek
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Chemestry
Abstract
In this work, natural hydroxyapatite (HAp) was extracted from bio-waste namely
caprine (goat) bone by two different methods such as thermal treatment only and
chemical method followed by thermal treatment. Thus produced HAp was
characterized by different scientific tools like XRD, FTIR and SEM analyses.
The specific surface area of HAp was found to be 144.89 m
viii
2
/g measured by using
methylene blue adsorption method. Similarly, pHPZC
value was calculated as 7.2
determined by using pH drift method. Adsorption study of different microorganisms on
the extracted HAp as a functions of various parameters like concentration of adsorbate,
contact time and adsorbent dose was studied. The maximum adsorption efficiency at
pH 7 for Escherichia coli, Candida albicans, Staphylococcus aureus and Acinetobacter
baumanii was found to be 32.57 × 10
5
, 37.17 × 10
5
, 6.7 × 10
5
and 43.47 × 10
cfu/mg
respectively from Langmuir adsorption isotherm model. The contact time required for
maximum adsorption fell within 25 mins. for all microorganisms. The experimental
data was best fitted with pseudo-second order kinetics model.
HAp showed the antimicrobial activity only upon Acinetobacter baumanii among the
studied microorganisms. MIC and MBC values of HAp were found to be 6.25 and 100
mg/mL respectively against Acinetobacter baumanii. Thus, hydroxyapatite was
synthesized from bio-waste caprine (goat) bone which can be used as an adsorbent in
microbial adsorption and an antimicrobial agent.
Keywords: Natural hydroxyapatite, Bio-waste, Adsorption, Microorganisms,
Antimicrobial activity
Description
Keywords
Natural hydroxyapatite, Bio-waste, Bio-waste, Adsorption