Microflora on Black Gram [Vigna mungo (L.) Hepper] Seeds from Different Storage Condition
Date
2018
Authors
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Publisher
Department of Botany
Abstract
lack gram is a most important summer pulse, which has a great market demand with a good
potentiality for trade and export in Nepal. The production of black gram has ceased at higher
rate by many fungi, as fungi infest in seeds. In this research, contact and systemic fungicides
were applied in different concentrations to prevent its post-harvest lose.
Seeds of black gram from two different storage sources were tested for seed-borne fungi.
PDA, Blotter and Sand methods were used to isolate seed-borne fungi, in which PDA method
was found to be the best. Seeds from traditional storage were found to be more infested with
seed-borne fungi in comparison to seeds from the market. Among 25 isolated fungi species,
four dominant species such as Aspergillus flavus, Aspergillus niger, Alternaria alternata and
Fusarium sp were taken for treatment with chemical fungicides. Aspergillus flavus and
Aspergillus niger were best controlled by Carbendazim, and Fusarium sp. and Alternaria
alternate were best controlled by Mancozeb. Carbendazim was found to be most effective at
the concentration of 1600 ppm. These chemicals have shown good inhibitory action against
fungi. In addition to this, seeds were treated with plant extracts of Azadirachta indica and
Ageratum haustonianum to reduce the presence and frequency of pathogens. Such seed
treatment showed effective control over different selected seed-borne fungi. Plant extracts,
which are residue free products and pose lower risk to pollution, were found to be good for
reducing the pathogens.
Keywords: Synthetic, Fungicide, Frequency, Pathogen
Description
Keywords
Synthetic, Fungicide, Frequency, Pathogen