Preparation of amperometric glucose biosensor by means of electropolymerisation of polyaniline onto graphite
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Chemistry
Abstract
In this study, an amperometric glucose biosensor with immobilization
of glucose oxidase enzyme on electrochemically polymerized polyaniline film
onto graphite surface has been prepared. The immobilization of the enzyme
glucose oxidase has been accomplished via the physical entrapment technique.
Electropolymerization of aniline onto the suface of the graphite was carried
out by cyclic voltammetry (potential range of -0.4V to 1.2V, scan rate
100mV/sec, 10 cycles) by using standard calomel electrode (SCE) as
reference and platinum wire as counter. Characterization of the PANi/Graphite
was carried out with the aid of cyclic voltammetry in 1M HCl, obtaining three
couple of peaks from which the two pair were distinct, having proved the
electro-activity of the PANi film. The enzyme was potentiostatically
immobilized onto the surface from 0.1M phosphate buffer of pH
6.0 with
200U/mL glucose oxidase (GOx) enzyme. The amperometric response was
studied by using hydrodynamic amperometric mode. Determination of glucose
was carried out by the measurement of amperometric response obtained during
the reduction of enzymatically produced hydrogen peroxide at - 0.4 V vs
calomel electrode and platinum wire as counter in phosphate buffer of pH 6.0.
Biosensor was found to be responsive within the concentration range of
0.01ML
-1
-0.1ML
-1
. All experiments were carried out at room temperature.
Description
Keywords
Glucose biosensor, Electrochemical synthesis