EFFICACY OF HAND SANITIZERS AGAINST STANDARD BACTERIAL CULTURES
Date
2022-08-08
Authors
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Publisher
Amrit Campus
Abstract
ह्याण्ड स्यानिटाइजरहरु हातको स्वच्छता कायम राख्न प्रयोग गरिन्छ । कोरोना महामारीले
यसको प्रयोग बारे ध्यान खिचेको थियो । धेरै कम्पनीहरुले उत्पादन गरिरहेका छन् तर
प्रभावकारीता राम्रोसँग स्थापित हुन सकेको छैन । यस अध्ययनको मुख्य उद्देश्य काठमाण्डौमा
बिक्री हुने ह्याण्ड स्यानिटाइजरहरुको व्याक्टेरियालाई मार्ने क्षमताको प्रभावकारीता मूल्याङ्कन
गर्नु थियो । यस अध्ययन मार्च देखि अप्रिल २०२२ सम्म गरिएको थियो । यस अध्ययनमा
काठमाण्डौका बजारबाट ३१ वटा अल्कोहल आधारित ह्याण्ड स्यानिटाइजर किनिएको थियो । Hand sanitizers are used to maintain hand hygiene. Corona pandemic had gain the
attention; many companies are producing it and are marketed but their efficacy have not
been well established. Thus, the main aim of this study was to evaluate the antibacterial
efficacy of the hand sanitizers sold in Kathmandu. This study was carried out from March
to April, 2022. In this study, 31 alcohol based hand sanitizers were purchased from the
markets of Kathmandu. Among them, 15 liquid based hand sanitizers (9 contain alcohol
and 6 contain alcohol with additional ayurvedic ingredients) and among 16 gel based
hand sanitizers, (10 contain alcohol and 6 contain alcohol and additional ayurvedic
ingredients). Efficacy of hand sanitizers were evaluated using standard ATCC cultures:
Escherichia coli 35218, Escherichia coli 25922, Staphyloccoccus aureus 43300 and
Klebsiella pneumoniae 700603 by agar well diffusion method. In the volume of 50 μl
hand sanitizers, (46.67%, 60%, 26.67% and 33.33%) liquid based, (20%, 40%, 33.33%
and 26.67%) liquid with ayurvedic ingredients based, (12.5%, 18.75%, 18.75% and
12.5%) gel based and (12.5%, 12.5%, 12.5% and 6.25%) gel with ayurvedic ingredients
based hand sanitizers showed the antibacterial effect against E. coli 35218, E. coli 25922,
S. aureus 43300 and K. pneumoniae 700603 respectively. In the volume of 100 µl hand
sanitizers, (53.33%, 60%, 46.67% and 46.67%) liquid based, (40%, 40%, 40% and 40%)
liquid with ayurvedic ingredients based, (50%, 62.5%, 62.5% and 62.5%) gel based and
(25%, 31.25%, 25% and 25%) gel with ayurvedic ingredients based hand sanitizers
showed the antibacterial effect against E. coli 35218, E. coli 25922, S. aureus 43300 and
K. pneumoniae 700603 respectively. In the volume of 150 µl hand sanitizers, (60%, 60%,
60% and 60%) liquid based, (40%, 40%, 40% and 40%) liquid with ayurvedic
ingredients based, (62.5%, 62.5%, 62.5% and 62.5%) gel based and (31.25%, 31.25%,
31.25% and 31.25%) gel with ayurvedic ingredients based hand sanitizers showed the
antibacterial effect against E. coli 35218, E. coli 25922, S. aureus 43300 and K.
pneumoniae 700603 respectively. Comparatively, liquid with ayurvedic ingredients
based sanitizers were more effective than gel with ayurvedic ingredients based sanitizers.
Description
Keywords
Agar well diffusion method, Antibacterial effect, ATCC Cultures, Hand sanitizers.