Biological and Phytochemical Studies on Some Medicinal Orchids of Nepal
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Department of Chemistry
Abstract
Biological and phytochemical screenings of some orchid species growing in Nepal were
studied. The ethanolic extracts of orchid species were tested against both gram positive
and gram negative standard bacterial strains viz. Vibrio cholerae, Staphylococcusaureus,
Klebsiella pneumoniae, Bacillus subtilis and Escherichia coli. All orchid extracts showed
good bacterial Zone of Inhibition(ZOI) against Staphylococcus aureus. The crude
extracts of Pholidota imbricata and Coelogyne cristatawere shown highest activity
against Vibrio cholerae (ZOI -14 mm) and Staphylococcus aureus(ZOI –14 mm)
respectively. The MIC and MBC value of the extracts of Pholidota imbricata and
Coelogyne cristata were found to be 62.5 mg/ml and 31.25 mg/ml, 125 mg/ml and 250
mg/ml respectively. Only Pholidota imbricata and Pholidota articulata extracts were
shown fairly good activity but all others extracts were shown very less activity or even
failed to show any activity against fungal organisms viz.Candida albicans, Rhizopus
stolonifer and Mucor spp. From the ethanolic extracts of orchid species,
phytocon stituents detected qualitatively were Alkaloids, Terpenoides, Flavonoides,
Tannins, Reducing sugars and Cynonogenicglycosides.