Studies on Antioxidant and Xanthine Oxidase Inhibitory Activities of Coccinia Grandis (L.) Voigt, Datura Metel (Linn.) and Tagetes Patula (L.) from Rupandehi District of Nepal
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Department of Chemistry
Abstract
Nepal is great country having all the types of biodiversity which is suitable for
the growth of different type plants. Different types of natural products and their
biological activities can be obtained from these plants which could have great
benefits in cure of different diseases. Phytochemical screening of different plant
extracts showed the rich resources of secondary metabolites such as alkaloids,
flavonoids, phenols etc. Calculations of TPC and TFC value of plant extracts
showed that T. patula content highest value TPC (630.839±2.633 mgGAE/g)
and TFC (79.605±1.808 mgQE/g) respectively. Antioxidant activities of these
plant extracts studied by both DPPH and FRAP both showed D. metel with IC50
value of 0.039 mg/mL and 357.0365±0.682317 mm Fe (II)/L respectively, as a
potent antioxidant among the selected three plant extracts. The antibacterial
activity was studied by well diffusion method which showed plant extract of T.
patula only showed the activity of 10 mm ZOI value at concentration of 50
mg/mL as compared to standard drug against S. aureus while other plant
extracts did not showed any activity against both gram positive and gram
negative bacteria. Xanthine oxidase (XO), an enzyme widely distributed among
mammalian tissues, is associated with the oxidation of xanthine and
hypoxanthine to form uric acid. This is the serious effect on the human being
many people are suffering from this disease. The antigout activity of these plant
extracts were studied by xanthine oxidase inhibition assay where methanolic
extract of C. grandis with IC50
value 0.491 mg/mL showed the highest xanthine
oxidase inhibition activity among selected plants, refers it as potent antigout.
Since C. grandis was selected for column and FTIR analysis of different fraction
was observed. FTIR analysis showed different carbonyl, phenolic, alkane
compounds are present in the extracts. This study may provide scientific basis
for the use of selected plants and may provide valuable information for further
research.