Medically Important Vibrios from Different Environmental Water Samples from Eastern Nepal
Date
2016
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Faculty of Microbioloy
Abstract
Safe drinking water, as defined by the guidelines, does not represent any
significant risk to health over a lifetime of consumption. The most important
bacterial diseases transmitted through water are cholera, typhoid fever and
bacillary dysentery, focusing on the biology and ecology of the causal agents
and on the diseases‟ characteristics and their life cycles in the environment.
Diarrhoea, which is caused by poor sanitation, hygiene and water quality, is
one of the most prevalent water borne disease in Nepal. This study was
performed from June 2016 to November 2016. The aim of research was to
evaluate significant of vibrio in environmental water in eastern regions of
Nepal (especially around Dharan and Ithari). Altogether 60 water samples
were collected and were incubated in alkaline peptone water and cultured in
TCBS agar.Identification of the significant isolates was done by standard
microbiological techniques and antibiotic susceptibility testing was done by
Kirby Bauer disc diffusion method.The seven different species of vibrios
isolated were V. cholerae (22.2%), V. parahaemolyticus (19.5%), V. furnissii
(16.7%), V. vulnificus (16.7%), V. fluvialis (13.9%), V.alginolyticus (5.5%)
and V. metschnikovii (5.5%). Vibrios isolates were 100% sensitive to
Amikacin, Azithromycin, Ceftriaxone, Chloramphenicol, Doxycyclin,
Imipenem, Levofloxacin, Norfloxacin and were varying degrees of resistance
to Ampicillin, Ceftazidime, Cefotaxime, Ciprofloxacin, CoTrimoxazole,
Erythromycin, Gentamicin, Nalidixicacid, Nitrofurantoin, Ofloxacin and
Tetracycline. Among the isolates, Vibrio Cholerae was found to be higher
percentage. Therefore, monitoring of V. cholerae in the environmental water
samples in the interim period between cholera epidemics is of great
significance for early warning of an outbreak, as well as prevention and
control of cholera epidemics when they occur.
Description
Keywords
Drinking water,., TCBS agar, Alkaline peptone water