Isolation, Charectarization and Study of Biological Activities of four Poisonous Medicinal Plants of Chitwan, Nepal

Abstract

Four poisonous medicinal plants species, namely; Calotropis gigantea,Ageratum houstonianum, Catharanthus roseus, The vetia peruviana (seed), and The vetia peruviana(leaves) have been collected from the central Nepal, Chitwan and the crude methanol extract of respective plants were studied for their phytoconstituents and biological activity. Phytochemical screening showed thepresence of glycosides, flavonol glycosides and coumarin glycosides as richcomponents. The Brine-shrimp bioassay of various plants extract showed that C.gigantean,A. houstonianum and C.roseusexhibited high toxicity against brine shrimpnauplii at LC (μg/ml) values of 23.44, 27.54 and 83.17 respectively. In addition to this, study of antimicrobial activity of respective plants extract on methanol showedthat,all the four plants species were highly active for Staphylococcus aures and C.gigantea is pharmacologically active for other bacteria suchE. coli, S. aures, K.oxytoca and P. aeruginosa. Study of anti-cancer activity on human pancreatic cancercells such as PANC-1 revealed that the preferential cytotoxic activity of crudemethanol extract of C. giganteawas higest at 100μg/ml for both NDM and DMEM.Column chromatography of methanol extract of aerial parts of C. gigantea has resulted the isolation of two pure compounds CG 50 21 , and CG The compoundCG 21 22 . was suggested as β-sitosterol by comparing TLC, IR and melting point with authentic sample.