Microbiological Study of Urinary Tractinfection in Patient Visiting Paropakar Maternity and Women’shospital, Thapathali, Kathmandu

Abstract

This study was conducted in patients visiting Paropakar Maternity and Women’sHospital, Thapathali, with an objective to assess the Microbiological study of UrinaryTract Infection.Annually,women and their neonates are in the risk of morbidity andmortality due to UTI in case of Nepal. In this study, atotal of 1246 clinical samples ofurine were collected. Prevalence of uropathogens was found 29.61% (369/1246). Atotal of 369 uropathogens of which 8 species from Gram negative (82.66%) and 4species from Gram positive (17.34%) were isolated. In Gram negative, the mostpredominant was E. coli (62.3%) followed by Klebsiella pneumoniae (6.5%),Proteus mirabilis(4.9%),K. oxytoca(3%),P. vulgaris(2.7%),Enterobacter cloacae(0.5%) andCitrobacter freundii (0.5%).From Gram positive most prevalent wereStaphylococcus aureus(7.6%)andcoagulase negative staphylococci (CoNS)(7.6%)followed by Streptococcus spp. (1.4%) and S. fecalis (0.8%). The most efficientantibiotics in Gram negatives were found Tobramycin (87.54%). InS. aureusAmikacin(96.43%) was found more susceptible whereas for CoNS and Streptococcus spp.maximal efficient antibiotic was found Nitrofurantoin (75%). Altogether 199 Multidrugresistant strains were isolated. Highest percent of MDR were found inK. pneumoniae(58.33%) in Gram negatives andS. aureus(82.14%) in Gram positives urine isolates, atotal of 14Methicillin Resistant Staphylococcus aureus(MRSA)were isolated. AllMRSA isolates were found to beVancomycin sensitive. Mutiple Antibiotics Resistanceindex (MARI) of isolates was found more than 0.20 in 247 (66.93%) of isolates. MARIof antibiotics was found highest in antibiotics Nalidixic acid in Gram negative andMoxifloxacin in S. aureusand Penicillin G in CoNS andStreptococcusspp. Previousantibiotics use was found significantly associated with MDR isolates (p=0.029;OR=1.752 CI=1.159 and 2.650). Keywords:host factors, MARI, uropathogens