Bacterial Isolates from Ear Discharge of Patients suffering from Otitis Media and Their Antibiotic Susceptibility Pattern

Abstract

This study was conducted to identify the aerobic bacterial pathogen causing otitismedia and determine their antibiotic susceptibility pattern. Aural discharge samples were collected from the patients visiting the Ear Nose ThroatOut Patient Department of Shree Birendra Hospital, Chhauni, who were diagnosed with chronic suppurative otitis media and acuteotitis media. Standard microbiological procedures were employed to isolate and identify the causative agents of the infection and determine the antibiotic susceptibility pattern of isolated pathogen. Among total 153 samples,128(84.31%) samples were positive.119(77.7%)samples produced single organism and9(5.8%) samples gave mixed growth.The total of 136 isolates was obtained. Gram positive organisms (64%) were predominant. The most common isolates were Staphylococcus aureus from70(51.5%) cases followed by Pseudomonas aeruginosa from 27 (19.9%) cases.Other organisms isolated were Coagulase negative staphylococci (11.8%), Klebsiella species (6.6%),Escherichia coli (5.1%), Citrobacter fruendii (2.9%),Proteus mirabilis (0.7%), Streptococcus pyogenes (0.7%) and Haemophilus influenzae(0.7%).S. aureus showed greater sensitivity(67.1%) to cefoxitin than other antibiotics tested. Antibiotic susceptibility test of P. aeruginosa showed100%sensitivity to imipenem, 92.6% to piperacillin, 88.9% each to ceftazidime and amikacin and 74.1% to ciprofloxacin. Similarly, most E. coliisolates (71.4%)were sensitive to gentamicin, C. fruendii (100%) to gentamicin, Klebsiella sps(77.8%) to ciprofloxacin and gentamicin each.S. aureuswas found to be the most common etiological agent of otitis media followed byP.aeruginosa. Keywords:Otitis media, Chronic suppurative otitis media,S. aureus,P.aeruginosa