BIOLOGICAL ACTIVITIES OF CALLICARPA MACROPHYLLA VAHL. LEAF EXTRACT AND ITS HRLCMS BASED PROFILING
| dc.contributor.advisor | Bodh Banu Bhattarai,Ph.D. Asst. Prof. | |
| dc.contributor.author | THAPA, SABI | |
| dc.contributor.author | Co Supervisor Ganga Raj Pokharel, Ph.D. (Associate Prof.) | |
| dc.date.accessioned | 2025-03-24T06:20:08Z | |
| dc.date.available | 2025-03-24T06:20:08Z | |
| dc.date.issued | 2023-10-11 | |
| dc.description.abstract | Callicarpa macrophylla Vahl. is a traditional medicinal plant that belongs to Verbaceaceae family growing in Nepal, India, Bhutan and Sri Lanka. The leaves of Callicarpa macrophylla were collected from Tanahun district in the month of October. The leaves were then air dried, crushed into powder using kitchen mixer. The powdered sample was extracted with methanol by maceration, concentrated using Rota vapor and analyzed the concentrated extract for the presence of different bioactive compounds, total phenolic content (TPC), total flavonoid content (TFC), total antioxidant activity (TAC), effectiveness against microbes along with HR-LCMS analysis. The antibacterial activity was tested against 6 bacterial strains like B.subtilis, S.aureus (gram positive strains) and K.pneumonia, E.coli, S.typhi, P.aureginosa (gram negative strain). The TAC was quantitated using Phosphomolybdenum assay. TPC and TFC were evaluated using Folin- Ciocalteu reagent assay and Aluminum Chloride assay methods, respectively. The semi-solid methanol extract of leaves of the C.macrophylla obtained was 10.8% w/w of the dried powder. The qualitative analysis showed the presence of alkaloids, flavonoids, phenols, saponins, amino acid, coumarins, gums and resins, fixed oils and fats. The anti-bacterial test showed the following inhibition zones: B.subtilis- 17.333 mm, S.aureus- 14.167 mm and K.pneumonia- 9.667 mm but no activity was shown in E.coli, S.typhi, and P.aeruginosa. The TPC was observed to be 43.489±0.175 mg GAE/g of dry material while TFC was 189.8287±0.429 mg RE/g of dry material. The TAC was found to be 63.953±0.271 mg AAE/g of dry material.The HR-LCMS analysis showed total of 70 known compounds and 5 unknown compounds. Some of the useful phytochemicals observed were Trimeprazine, Methotrimeprazine, Flurandrenolide Oxazepan, Sakacin, Antimycin A, Ritterazine A, Pleuromutilins etc. The most abundant compound was Trimeprazine, an antipruritic and analgesic agent. These compounds are of high medicinal value and employed in the treatment of various cancers (colon, prostate, breast), antibacterial diseases, multiple sclerosis, seizure disorder, inflammation, swelling and also used as sedative. Since ancient, the plant has been used as remedy by local people to treat different disorders. The HR-LCMS data provided the evidence of the presence of different medicinal bioactive constituents in the plants, which was further assisted by antibacterial activity, TPC, TFC and TAC values. However, it also contained some unknown compounds that may have remedial properties suggesting the further analysis of these compounds. Keywords: phytochemical, antioxidant, antimicrobial, phenolic, flavonoid, HR-LCMS | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14540/24653 | |
| dc.language.iso | en | |
| dc.publisher | Department of Chemistry, Birendra Multiple Campus | |
| dc.title | BIOLOGICAL ACTIVITIES OF CALLICARPA MACROPHYLLA VAHL. LEAF EXTRACT AND ITS HRLCMS BASED PROFILING | |
| dc.type | Thesis | |
| local.academic.level | Masters | |
| local.institute.title | Birendra Multiple Campus, Bharatpur |