ANTIOXIDANT AND BIOLOGICAL EVALUATION OF METHANOLIC, ETHYL ACETATE AND DICHLOROMETHANE EXTRACT OF Zingiber officinale COLLECTED FROM TWO DIFFERENT DISTRICTS OF NEPAL
Date
2025-02-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Chemistry, Birendra Multiple Campus
Abstract
Antioxidant and biological evaluation of methanolic, ethyl acetate, and
dichloromethane extract of Zingiber officinale collected from two different
districts of Nepal is not clearly understood yet. This study investigates these
properties in ginger extracts from Arghakhanchi and Dang districts. The ginger samples
were gathered, dried, and extracted using the cold percolation method with three
solvents: methanol (MeOH), dichloromethane (DCM), and ethyl acetate (EtOAc). Total
phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity were
measured via DPPH assay, antimicrobial activity, and toxicity using a brine shrimp
bioassay. The methanolic extract from Dang district (S2(MeOH)) showed the highest
TPC (123.02 ± 2.84 mg GAE/g) and TFC (25.42 ± 1.69 mg QE/g), with strong
antioxidant activity (IC50 = 162.73 ± 3.22 μg/mL). The dichloromethane extract from
Arghakhanchi (S1(DCM)) had the lowest TPC (36.23 ± 3.45 mg GAE/g) and TFC
(14.32 ± 1.74 mg QE/g), with weaker antioxidant activity (IC50 = 279.63 ± 14.67
μg/mL). The ethyl acetate extract from Dang (S2(EtOAc)) showed the best
antimicrobial activity, especially against S. aureus and P. aeruginosa. The methanolic
extract demonstrated strong antifungal properties. Brine shrimp bioassay results
indicated higher toxicity in the methanolic extract (LC50 = 3.0 × 10³ μg/mL). Ginger
from Dang district showed the most promising antioxidant and antimicrobial properties.
Further research on active compounds is recommended for potential medicinal use.
Keywords: Antimicrobial, Antioxidant, Ginger (Zingiber officinale), Total Flavonoid
Content, Total Phenolic Content.
Description
The thesis work "Antioxidant and Biological Evaluation of Methanolic, Ethyl
Acetate and Dichloromethane Extract of Zingiber officinale Collected from Two
Different Districts of Nepal", submitted by Purnima Banjade as a part of M.Sc.
Coursework in Chemistry at Birendra Multiple Campus is carried out under my
supervision. Any part of this thesis work has not been submitted for any other degree
award.