Antidiabetic activity of mahonia nepaulensis DC in neonatal Streptozatocin induced type-II Diabetic model rate

Abstract

Diabetes mellitus, is a group of metabolic disorders characterized by a high blood level over a prolonged period of time. It is due to either the pancreas not producing enough insulin, or the cells of the body not responding properly to the insulin produced. Symptoms often include frequent urination, increased thirst and increased appetite. Serious long-term complication include cardiovascular disease, stroke, chronic kidney disease, foot ulcers, damage to the nerves, damage to the eyes, and cognitive impairment. Diabetes management concentrates on keeping blood sugar level as close to normal, without causing low blood sugar. This can usually be accomplished with dietary changes, excessive weight loss and use of appropriate medication (insulin, oral medication) The main aim of this research work is to evaluate the antidiabetic activity of methanolic extract from Mahonia nepaulensis bark in n-STZ induced type-II diabetic model rats. The oral glucose tolerance test was performed in normal as well as diabetic rats. Experimental rats were divided into four groups (n=6) as group-I control received normal water (10 mL/kg/bw), Group-II control received diabetic water (10 mL/kg/bw), Group-III standard received Gliclazide (20 mg/kg/5 mL/bw) and Group-IV received methanol extract (80%) of stem bark of Mahonia nepaulensis (MNB) at a dose of 1.25 g/kg/10 mL/bw. The blood was withdrawn from tail the vein at a different time intervals of 0, 30, 60, and 90 minutes again glucose levels were measured using glucose oxidaseperoxidase reactive strips and glucometer.

In the oral glucose tolerance test MNB group showed significant decrease in blood glucose level. In STZ induced diabetic rats fasting blood glucose levels of the treatment group were significantly reduced by 28 days of treatment with MNB extract. The isolated compounds from M. nepaulensis include β-Sitosterol, Berberine, and 5,6Dihydro-9,10-dimethoxy benzo-1,3-benzodioxolo 5,6-quinolizinium.

The crude extracts of Mahonia nepaulensis bark have potent antidiabetic activity and antilipidemic activity in streptozotocin-induced type-II diabetic rats. Keywords: Mahonia nepaulensis bark, OGTT, Gliclazide, Streptozotocin, Glucometer