Detection of metallo beta-lactamases producing pseudomonas aeruginosa from different clinical samples

Abstract

Pseudomonas aeruginosais an opportunistic bacteria that have an ability to cause nosocomial infection in patient with diverse range of infection such as urine infection, respiratory infection, bacteremia, wound and other central nervous system. Increase in antibiotic resistant has been a nuisance in the hospital settings. More ever, increase in prevalence of metallo beta lactamase producing P. aeruginosa leads to increase in morbidity and mortality due to the limited number of choice of drugs for treatment. This study aims in isolating the P. aeruginosa from different clinical samples and detection of multidrug resistant as well as screening of MBL producing P. aeruginosa. The hospital based cross sectional study was done in Grande International Hospital, Kathmandu from January – June 2024. A total of 1500 different clinical samples such as urine, blood, sputum, pus, tissue, wound etc. were processed during the study time, where 126(8.2%) of Pseudomonas aeruginosa were isolated from different samples of different ages and gender, of either inpatient or outpatient. 34.6% isolates were isolated form urine,31.7% from sputum, 19% from blood, 1.6% from pus, 4.8% from wound swab and 0.8% form tissue were obtained from 66.1% of male and 38.9% of female infected patients. MBL prevalence was found to be 16.6% which were more from ICU (47.6%/) followed by 33.3% from general ward. Levofloxacin had higher sensitive pattern(82.4%) and Nitrofurantoin was more resistant (90.2%).In MBL isolates, Aztrenam was found to be least resistant (33.3%). Infection by P. aeruginosa can cause a medical threat. A multidrug resistant isolates can even be more difficult to treat. Increased MBL isolates has been a nuisance in hospital settings. Therefore, routine evaluation should be done to ensure the control of these isolates.

Key words: Pseudomonas aeruginosa, Multidrug resistant,Metallo betalactamases (MBLs),