IsolationofSalmonella Spp. in different meat samples of Kathmandu valley
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Faculty of Microbiology
Abstract
The present study was conducted at Central Veterinary Laboratory, Tripureshwor,kathmandu from Ashoj to Mangsir, 2065 with the aims of determination of theisolation ofSalmonella spp. in meat samples of kathmandu Valley. Altogether 250meat samples including Goat (75), Chicken (125), and Buffallo (50) were collectedfrom different retail shops of kathmandu Valley. All these samples were processedusing standard procedures for the isolation and identification ofSalmonella spp.frommeat samples. All Salmonella isolates were tested to different antibiotics usingstandard protocol for the determination of antibiotic susceptibility profile.
The isolation ofSalmonella spp. detected case was found to be 9.2%. The detectionrate was found highest in the month of Ashoj (11.11%).The isolation of Salmonellasppdetected case in retail shops of kathmandu (11.01%) was higher than that ofBhaktapur (8.77%) and Latitpur (6.66%). The isolation ofSalmonella sppdetected inchicken (11.2%) was higher than buffalo (8%) and goat (6.66%) meat samples.Among 23 isolates of Salmonella, 39.13% were S. gallinarum, 34.78% were S.pullorum and 26.08% were Salmonella spp. belonging to Serogroups D and E.Among these serogroups, Salmonella belonging to serogroup D was the mostpredominant. Multidrug- resistant (MDR) was observed in only 4.34% of theSalmonella isolates. However, most of the isolates in addition to those pathogenic tohuman ( i.e , belonging to Serogroup D and E) were resistant to the antibioticsTetracycline and Nalidixic acid. All isolates were sensitive to Cephotaxime,Ciprofloxacin, Ofloxacin, and Chloramphenicol.
Key words: Salmonella, Serogroups, Multidrug-resistant, Pathogenic, Antibiotics.