NUTRIENT ELEMENTS AND HR-LCMS ANALYSIS OF SECONDARY METABOLITES PRESENT IN METHANOLIC EXTRACTS OF Colocasia esculenta (L.) SCHOTT (cocoyam)
Date
2025-02-06
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Department of Chemistry, Birendra Multiple Campus
Abstract
Nutrient Elements and HR-LCMS Analysis of Secondary Metabolites Present in
Methanolic Extracts of (Colocasia esculenta (L.) Schott (Cocoyam) is still not
understood clearly. Methanolic extracts of three different parts (tuber, petiole and
leaf) of plants from different sampling spots (Palung, Upperdangadhi and Padampur)
were utilized to assay antioxidants activities through 2, 2-Diphenyl-1-picrylhydrazyl
(DPPH) free radical scavenging assay and phosphomolybdate assay (TAA), total
phenolic (TPC), total flavonoid (TFC) and total carbohydrate content (TCC) using
spectrophotometer (T80+, PG Instruments, UK). The antimicrobial susceptibility test
of methanolic extracts of plant samples was conducted on seven microorganisms i.e.
E. coli, K. pneumonia, P. aeruginosa, B. subtilis, S. aureus, A. baumannii and S.
typhi, Pseudomonas and Salmonella species. Nutrient elements and toxic metals
quantification and HR-LCMS analysis of plant tuber, petiole and leaf from
Upperdangadhi were carried out at Centre for Research in Nanotechnology and
Science (CRNTS), Sophisticated Analytical Instrument Facility (SAIF), Indian
Institute of Technology, Bombay, 400076, India, using ICP-AES and HR-LCMS
spectroscope model G6550A respectively.IC50 value of plant extracts was found in between 240.45-1018.44 µg/mL. TAA by
phosphomolybdate assay was found 1.25±0.01 to 28.29±0.01 mg AAE/g DS. TPC
was found in between 6.38±0.004 and 19.96±0.01 mg GAE/g DS. TFC was found in
the range of 10.46±0.0007 and 89.1±0.006 mg QE/g DS. TCC was found in between
1.78±0.0 and 112±0.0 mg GE/g DS. Concentrations of elements were found in the
order of tuber, petiole and leaf as: Ca (8910.0, 303.0, 355.0 mg/Kg), Cr (0.476,
0.252, 0.427 mg/Kg), Cu (1.226, 0.670, 0.727 mg/Kg), Fe (15.6, 1.241, 2.41 mg/Kg),
Mg (96.8, 43.6, 104.0 mg/Kg), Zn (128.0, 0.685, 1.942 mg/Kg) and As (15.7, 9.8,
65.6 mg/Kg). Concentration of Pb was below the detection limit(1ppb).HR-LCMS analysis of tuber, petiole and leaf revealed that the extract contained 200,
140 unidentified, 60 identified- 11 alkaloids, 4 phenolic, 5 flavonoids, 3 glycosides, 5
steroids, 32 others, 200, 85 unidentified, 115 identified- 12 alkaloids, 15 phenolic, 26
flavonoids, 9 glycosides, 53 others, 197, 106 unidentified, 91 identified- 13 alkaloids,
17 flavonoids, 3 terpenoids, 1 steroid, 12 pharmaceutical drugs, 45 others
respectively. Neuraminic acid, Amprenavir, Butin, catechin, cyclopamine,disopyramide, Funtumine, gentamycin, Jervin, Kaemferol, ketamine, retapamulin,
swertiamarin, syringic acid, usambarensine etc. are potentially bioactive.
Key words: Cocoyam, Phytochemical screening, Antioxidant activity, Antimicrobial
Activity, DPPH, HR-LCMS
Description
On the recommendation of Ganga Raj Pokhrel, Ph.D. (Assoc. Prof.) and Bodh
Babu Bhattarai, Ph.D. (Asst. Prof.), this M.Sc. thesis submitted by Ajaya Mahato
entitled “Nutrient Elements and HR-LCMS Analysis of Secondary Metabolites
Present in Methanolic Extracts of (Colocasia esculenta (L.) Schott (Cocoyam),” is
forwarded by Department of Chemistry, Birendra Multiple Campus (BMC) to the
office of Dean, IOST, T. U.