Antibacterial and Antioxidant Property of Selected Medicinal Plants of Daman VDC, Makawanpur District
Date
2016
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Department of Botany
Abstract
Altogether fifteen plants used to treat stomach troubles like indigestion, diarrhea and
dysentery were selected for the study purpose. The medicinal plants were extracted in
methanol by Percolation with intermittent Sonication method. Bergenia ciliata had
the highest yield (20.03%) while Cautleya spicata had the lowest yield (2.90%). The
methanol extracts of these plants were evaluated for antibacterial activity against
medicinally important bacteria viz. Bacillus subtilis, Pseudomonas aeruginosa,
Escherichia coli and Staphylococcus aureus and also evaluated for the antioxidant
activity. The in vitro antibacterial activity was performed by agar well diffusion
method.
Among the 15 plants tested, in the present study, all plants except one (Achyranthes
bidentata) showed activity against at least one bacterium. Bergenia ciliata, Coriaria
napalensis, Potentilla fulgens, Pyracantha crenulata, Pyrus pashia and Rubus
ellipticus inhibited all the tested bacteria at 50 µg/ml and 100 µg/ml. Lindera
neesiana inhibited all the tested bacteria at 100 µg/ml only. Callicarpa macrophylla
inhibited only 3 of the tested bacteria at 150 µg/ml while Galium aparine and Uraria
picta inhibited Gram +ve bacteria only. Adiantum philippense, Anemone vitifolia,
Campanuma paillida and Cautleya spicata inhibited Bacillus subtilis only. Most of
the extracts showed activity against Gram +ve bacteria than Gram -ve bacteria.
Highest phenolic content was obtained for Potentilla fulgens (90.73 ± 0.71 mg
GAE/g) while the lowest was shown by Achyranthes bidentata (2.21 ± 0.19 mg
GAE/g). Similarly, highest content of flavonoid was obtained for Adiantum
philippense (21.19 ± 0.28 mg QE/g dry plant) and Cautleya spicata (21.19±0.52 mg
QE/g dry plant) and lowest for Bergenia ciliata (1.78 ± 0.11 mg QE/g dry plant).
Antioxidant activity of the methanolic extract was determined using DPPH method.
Maximum IC
50
value was obtained for Achyranthes bidentata i.e. 127.55 and
minimum for Bergenia ciliata i.e. 40.56. Thus, Bergenia ciliata was considered to be
the best antioxidant among the sampled plant samples.
Description
Keywords
Antioxidant property, Medicinal plants