Antimicrobial assay of lactobacillus species against multigrug resistane bacteria
| dc.contributor.advisor | Dr. Shyam Prakash Dumre | |
| dc.contributor.author | Khadka, Rama | |
| dc.date.accessioned | 2024-08-30T03:48:52Z | |
| dc.date.available | 2024-08-30T03:48:52Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Lactic acid bacteria are the group of bacteria which are commonly found in soil and in the guts of animals and they are favorable for both human and animal health. In this study, Lactobacillus species were isolated and identify from soil of a cow farm and perform its antimicrobial activity against MDR bacteria from October 2024 to March 2024 in the microbiology laboratory of Padma Kanya Multiple Campus, Bagbazar, Nepal. A total of 30 soil samples were collected from 3 locations of farm of Kathmandu valley for enumeration and isolation of Lactobacillus species on selective media (MRS). Pure cultures were obtained through sub culturing on MRS media and further morphological as well as biochemical test was be carried to identify the isolated strains. For the confirmation of Lactobacillus species, Gram staining, motility test and catalase test were done. For the further analysis, fermentation test, growth on different temperature, different salt concentration and different pH were also done. Antibiotic susceptibility test (AST) of Lactobacillus species and test bacteria was done by a modified Kirby- Bauer method. The screening test for bacteriocin was done by dot plate technique method. Then, the extraction of bacteriocin from isolated Lactobacillus spp. was done by precipitation method and antimicrobial assay was done against MDR test bacteria. From this study, 10 Lactobacillus species were identified. In this study, bacteriocin like compound were extracted from Lactobacillus spp. were from 24hrs (2day), 48hrs (3day), 72hrs (4day), 96hrs (5days) and 120hrs (6days). However, antimicrobial activity showed against MDR bacteria only after 72hrs (4day), 96hrs (5days) and 120hrs (6days). From 96hrs (5days) of extraction of antimicrobial substance bacteriocin like compound was able to inhibit all test bacteria which were Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. The highest zone of inhibition was showed by BL10 isolate against B. cereus (30mm) followed by KL1 isolate against E. coli (21mm) and KL5 isolate against B. cereus (20mm). From this study, antimicrobial activity of bacteriocin like compound showed the antimicrobial activity against all MDR bacteria. So, next generation antibiotics can be prepared from bacteriocin like compound extracted from Lactobacillus spp. for targeting the multi drug resistant bacteria. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14540/22750 | |
| dc.language.iso | en | |
| dc.publisher | Institute of Science & Technology | |
| dc.subject | Cow farms | |
| dc.subject | soil | |
| dc.subject | Lactobacillus species | |
| dc.subject | AST | |
| dc.subject | Bacteriocin | |
| dc.title | Antimicrobial assay of lactobacillus species against multigrug resistane bacteria | |
| dc.type | Other | |
| local.institute.title | Institute of Science & Technology |