In Vitro Study of Neopicrorhiza Scrophulariiflora (Pennell) Hong

Abstract

Micropropagation of Neopicrorhiza scrophulariiflora (Pennell) Hong an important endangered medicinal plant, was taken for this investigation. The explants (root, internode, node and shoot tip) obtained from in vitro grown seeds were cultured on MS medium supplemented with different concentration of BAP and NAA. Seeds were sown on MS medium and 40% of seed germination was found after 4 weeks. Shoot tip explant gave the most satisfactory result for shoot multiplication. Shoot tip explant cultured on MS + BAP (1ppm) + NAA (0.5ppm) produced maximum but reduced shoots. These microshoots with callus when cultured on MS hormone free media produced elongated shoots. MS media with BAP (1ppm) and NAA (0.5ppm) was found to be best for callus induction and shoot multiplication whereas elongation of shoot was done in hormone free MS media. MS media without auxin and with auxins (IAA, NAA and IBA) were used to induce rooting. Among these, auxin free media induced longer roots without formation of callus at base of shoots whereas all other combinations with auxins produced basal callus and hairy roots. Rooted plantlets were transferred on the pot containing soil: sand: vermiculite in 1:1:1 ratio with high humidity.