Study on Essential Oil of Valeriana Jatamansii Jones
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Department of Botany
Abstract
In the present investigation, phytochemical screening, biological screening and the study of essential oil constituents of hairy roots produced in-vitro and in–vivo rhizomes of Valeriana jatamansii Jones. were carried out. Phytochemical screening of different samples showed the presence of fatty acids, reducing compounds, basic alkaloids, flavone glycosides,sterol and triterpenes. Biological screening carried out using brine–shrimp nauplii showed that the ethyl acetate fraction of in–vitro rhizomes as the most appropriate fraction for study of cytotoxic activities. The amount of essential oil was found the highest in the hairy roots produced in 0.5mg/lNAA ie.(0.80%) and the minimum in the rhizomes collected from pyuthan i.e (0.56%). A total of seventeen compounds present in the essential oil could be identified by gas chromatography coupled with mass spectroscopy (GC-MS) analysis followed by mass library search. Patchoulenone, 1,2–butyl octyl ester of benzene dicarboxylic acid and bis (2-ethyl hexyl) phthalate were found only in the oil obtained from hairy roots .