Biotechnology

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https://hdl.handle.net/20.500.14540/123

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Browsing Biotechnology by Advisor "Gaurishankar Manandhar"

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    Contraceptive effect of tinospora cordifolia stem extract in mice
    (2019) Rijal, Sarbesh; Gaurishankar Manandhar
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    Evaluation of contraceptive properties of medicinal plant dioscorea bulbifear by in vitro sperm function and in vivo fertilization assay techniques
    (2020) Sharma, Jayaswori; Gaurishankar Manandhar
    Dioscorea bulbifera is one of the medicinal plants which have shown to possess various medicinal properties like anti- tumor, anti - inflammatory, anti- arthritis, anti- fertility etc. D. bulbifera causes antifertility effect by suppressing ovulation and implantation of embryos in females. The main objective of the present study is to assess a direct contraceptive effect of Dioscorea bulbifera bulbil and tuber extracts by investigating in vitro sperm function assays and in vivo fertilization (IVF) techniques. Spectrometric analysis exhibited absorbance peak at 250 nm in both extracts. The tuber extract showed distinct shoulder peaks at 280 nm and 340 nm while such peaks were in conspicuous in the bulbil extract. Mouse epididymal spermatozoa were incubated in capacitation medium with various concentrations of extract. Spermatozoa were labeled with FITC peanut agglutinin (FPNA) or Coomassie to study extract induced acrosome reaction. The treated spermatozoa were stained with trypan blue or incubated with hypoosmotic medium to study viability and membrane damage. Both extracts caused significant acrosome exocytosis at very low concentrations. Comparatively, tuber extract resulted similar rate of acrosome loss as bulbil extract. D. bulbifera tuber extract also caused membrane damage, evaluated by trypan blue staining method and hypoosmotic swelling test. Viability loss was remarkably lesser than acrosome exocytosis. SDS PAGE was performed for protein profile analysis between the treated sperm sample and untreated control, from which we observed no remarkable change on sperm protein bands in the treated sperm cells. Finally, by performing in vivo fertilization test we have observed that the extract treated female mice had reduced litter size. These results suggest that D. bulbifera extract might effectively inhibit fertilization. The present research will help in characterizing potential contraceptive effect of Dioscorea bulbifera and in determining whether it could be used as a targeted and topical, contraceptive. Keywords: Dioscorea bulbifera, anti- tumor, anti- inflammatory, anti-arthritis, anti- fertility, in vivo fertilization, FITC peanut agglutinin, acrosome exocytosis, contraceptive
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