Biotechnology
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Item Enzymes in hibition and antioxidant assay of ethno medicinal plants of Nepal(2017) Pokharel, Rupesh; Rajani Malla,n this research, both chemical and biological assays performed. Terminalia chebula, Terminalia bellerica, and Eucalyptus alba were found to be effective against Staphylococcus aureus with the zone of inhibition 18, 11 and 7 ug/ml respectively, rest of the selected plants were not found to be active against Pseudomonas aeruginosa. Staphylococcus aureus has been found to cause different disease like bacteremia, endocarditis, meningitis, skin infection and toxic shock syndrome. Eucalyptus alba showed highest polyphenol content (138.86±9.62 mg GAE/g dry wt.) while flavonoid content were even lower than polyphenol content while flavonoid content were even lower than polyphenol content. Phytochemical screening showed presence of tannins, polyphenol, flavonoids, and cardiac glycosides. IC 50 value of antioxidant activity for most of the selected plants were below 50 µg/ml. Least IC 50 value was found to be of Terminalia chebula that is 19.63±0.89 µg/ml. Such results indicated the efficacy against disease due to free radical like rheumatic arthritis. In enzymatic assays, low IC50 values by Terminailia chebula, Ampelocissus divaricata, Terminalia bellerica, Eucalyptus alba, and Woodfordia fruticosa for inhibition of α-glucosidase and 15-lipoxygense indicated their efficacy against disease due to free radicals like diabetes. Result of this research awares us about the use of these medicinal plants. Keywords: Medicinal plants, phytochemical screening, antioxidant, antibacterial activity, enzymatic assays.Item Quest for antimicrobiol agent: from nanoparticle to computational biology (antimi crobial resistance element CFR as the target protein)(2024) Shrestha, Pooja; Alina Shri SapkotaRapid emergence of superbugs at an unprecedented rate is posing great risk to human health. To address this issue, a diversified integrated approach of antibiotic development, scope of nanoparticles and in silico potentiators screening has been explored in this study. In this regard computation biology approach has been taken in consideration for identification of ligands that could be co-administered with the present-day PhLOPS antibiotics, especially chloramphenicol. The screening parameters ADMET was employed to screen ligands with drug-likeness. In addition, the ligand needed to be non-inhibitory to hMAT1A and Cyp450 3A4 due to their role in immunity and drug metabolism, hence molecular docking with these proteins were performed prior to final docking against the resistance element Cfr. The lead compound ZINC72320745 with inhibitory binding affinity against bacterial Cfr enzyme responsible for chloramphenicol clearance in bacteria has a furan scaffold that suggests the saprophytic nature of the Streptomyces sps. could be explored further with furfural supplementation in the media. However, in this research, tannic acid incorporated modified media was introduced to isolate robust Streptomyces, due to its polyphenolic stress inducing nature. Further, antimicrobials (antibiotic and biogenic nanoparticles) were synthesized and tested against standard pathogens after UV-Vis spectroscopy characterization of silver nanoparticles. The results showed the greater pathogen inhibition with silver nanoparticles than its secondary metabolite. Moreover, the FTIR analysis of the nanoparticle revealed presence of phenol, vinylic ether and imine or oxime group in extract, indicating responsible for its antimicrobial as well as reducing property. The isolate I53 has been found clade forming with Streptomyces rubiginosohelvolus strain 183DZ from the phylogenetic analysis suggesting relatedness. Keywords: Streptomycetes, antibiotics, Silver nanoparticle, Molecular docking, Cfr, RlmN, UV-Vis spectroscopy, FTIRItem Investigation of Antioxidant Antimicrobialand toxicity activities of lichens from high Attitude regions Nepal(2012) Jha, Baidyanath Kumar; Tribikram BhattaraiAvailable in fulltextItem Nano-biochemical approach for ‘One Pot’ reduction of atmospheric N 2 and CO 2 for bio-fuel and bio-fertilizer(2018) Adhikari, Saraswoti; Pramod AryalAvailable in fulltextItem In silico mediated tweaking of streptomyces for antimicrobial production; cloning for Riboswitch mediated inhibitation(2019) Ghimire, Sita; Pramod AryalAvailable in fulltextItem Pharmacokinetic study and assessment of cytoking expression level elicited by a novel acinetobacter baumannii phage in mouse model(2019) Regmi, Madhav; Rajani MallaAvailable in fulltextItem Molecular characterization of classical swine ferer virus vaccine strain by partial genome sequencing and analysis(2019) Budha, Tika Bahadur; Krishna Das ManandharAvailable in fulltextItem Charaterization, whole genome analysis and application bacteriophages to control biofilm produced by charbapenem resistant bacteria prioritized by world health organization(2018) Parajuli, Apshara; Rajani MallaAvailable in fulltextItem Cloning of ddi (DNA damage inducible) gene in pfcenv 3\ v4 transection vectors and production of recombinant generation of antibodies(2019) Majhi, Bhagawati; Krishna Das ManandharAvailable in fulltextItem Valorization of industrial waste (whey ) microbial manupulation for industrilization(2022) Maidali, Devraj; Pramod AryalAvailable in fulltextItem In- vitro assay of bacteriophage against salamonella in raw food products(2021) Chapagain, Yujeen; Rajani MallaAvailable in fulltextItem Study on anti cancer activity of 2- pyridineformamide thiosemicarbazones and their copper (ii) complexex on Hela cells(2023) Khatiwada, Rabindra; Krishna Das ManandharAvailable in fulltextItem Charaterization of myxobacteria by using molecular tools and identification of gene responsible for anti- microbial activity(2019) Subedi, Surendra Kumar; Rajani MallaAvailable in fulltextItem Contraceptive effect of tinospora cordifolia stem extract in mice(2019) Rijal, Sarbesh; Gaurishankar ManandharAvailable in fulltextItem Degradation of potato peels using amyase and pectinase producing fungal strain in electrochemical cell and byproduct analysis(2022) Bhatt, Puja; Jarina JoshiAvailable in fulltextItem Theapeutic options of covid- 19 management; computational approach targeting main protease ( Mpro)(2022) Subedi, Samiran; Pramod AryalAvailable in fulltextItem Potato meristem culture to generate virus- free plantlets and optimization of callus induction, transformation and microtuberization parameters(2024) Mishra. Kunjan; Sarbesh Das DangolAvailable in fulltextItem In- silico analysis for host range identification for broad spectrum bacteriophage (phi 6 ) to control pseudomonas syringae and its pathovars(2024) Pokharel, Sujata; Rajani MallaAvailable in fulltextItem Study of contraceptive effects of medicinal plant artemisia vulgaris by in vitro mouse sperm functions test and in vivo fertilization techniques(2020) Bhandari, Sabina; Gaurishankar ManandharAvailable in fulltextItem Expression vector construction to study promoter function of pseudomonas aeruginosa coba gene; A B- galactosidase based reporter assay(2020) Pathak, Pooja; Pramod AryalAvailable in fulltext