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https://hdl.handle.net/20.500.14540/142

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    THE BIOLOGICAL PROPERTIES OF AEGLE MARMELOS (L.) CORREA AND HR-LCMS BASED METABOLITES PROFILING
    (Department of Chemistry, Birendra Multiple Campus, 2023-10-11) Giri, Swikar; Co-Supervisor, Bodh Baby Bhattarai, Ph.D.(Asst. Prof.); Ganga Raj Pokharel, Ph.D. , (Associate Prof.)
    The Biological Properties as well as HR-LCMS analysis was carried out on the methanolic extract of Aegle marmelos (L.)Correa Leaves.Extract of the leaves was prepared using Soxhlet extractor. The leaves of Aegle marmelos has been employed as therapeutics for various kinds of disease traditionally over the years. This study employed the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, a sustainable and cost-effective method, to assess the antioxidant properties of the plant extract. This plant extractwas evaluated across six biological samples, namely S. typhi, E.Coli, K. pneumonia, P. aureginosa, B. subtilis, and S. aureus, revealing a substantial inhibition of growth in all of these biological specimens. To investigate further insights into the plant extract,Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Antioxidant Activity (TAA) and DPPH scavenging activitiesweremeasured using a double-beam UV-visible spectrophotometer. This analysis unveiled the antioxidant and antimicrobial activitiesinherent in the selected plant parts. Additionally, High-Resolution Liquid Chromatography-Mass Spectrometry (HR-LCMS) analysis of the sample was carried out for the selected plant parts.TheRotary evaporator was used to evaporate the solvent from the extract and18.45% yield extract was obtained from the dried leaves. The methanolic extract was subjected on the analysis of Total Phenolic Content (TPC) (59.307 ± 1.058 mg GAE/g dryweight extract), Total Flavonoid Content (TFC) (132.8±1mg QR/g dry weightextract), Total Antioxidant Activity (TAA)(309.444±2.341mg AAE/g dryweight extract) and IC50 value of DPPH scavenging activity was found to be 238 μg/mL Notably, antibacterial activity of the extract against Bacillus subtilisand Salmonella typhiwas found to be with zone of inhibition (ZOI)of 26±4.242mm and 14±1.414mm respectively.Intriguingly, the High-Resolution Liquid Chromatography-Mass Spectrometry (HRLCMS) analysis of the crude extract identified a total of 60 compounds among them 45 are known and 15v are unknown compounds. Some of these compounds have potential applications. So that the plant extract can be used for the preparation of medicine and natural product-based therapeutics. 3-beta,6-betaDihydroxynortropane, Manumycin A, Cepharenthine, Ritterazine A, and Saphenamycin has potential effect for the treatment of diseases like leukemia, cell cancer, constipation, skin allergy, inflammatory diseases etc. To sum up, plant extract has 15 unknown compounds that may exhibit potential biological activities. So further works are necessary for the characterization of these unknown molecules and precisely disclose their biological activity. Keywords: HR-LCMS, Anti-microbial, TAC, TFC, TPC, Phytochemical screening.