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Browsing Syllabus by Author "Co Supervisor Bodh Babu Bhattarai , Ph.D., Asst. Prof."
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Item BIOLOGICAL ACTIVITIES OF EUPHORBIA HIRTA LINN. EXTRACT AND ITS COMPREHENSIVE SPECTROSCOPIC ANALYSIS(Department of Chemistry, Birendra Multiple Campus, 2023-10-11) Silwal, Sachin; Co Supervisor Bodh Babu Bhattarai , Ph.D., Asst. Prof.; Ganga Raj Pokharel, Ph.D. , Associate Prof.Biological activities of Euphorbia Hirta Linn. extract and its comprehensive spectroscopic analysis was carried out using its methanolic extract. Euphorbia Hirta has been used since a long ago as a traditional therapy in many diseases. Hence, biological activities of the plant extract were assayed using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) which is sustainable and affordable approach for the measurement of antioxidant properties. Plant extract was assayed on five biological samples, i.e. coli, K. pneumonia, P. aeruginosa, B. subtilis and S. aureus. In all biological samples, growth of the biological specimens was inhibited significantly. Total phenolic content (TFC), Total flavonoid content (TFC) and total antioxidant activity (TAA) was assayed using double beam UV-visible spectrophotometer. The analysis of the plant extract revealed the antioxidant, antimicrobial activities of selected plant part. The HR-LCMS analysis of the sample disclosed the phytochemical composition of selected plants parts. The gummy crude extract of the Euphorbia Hirta Linn. was found to contain diverse range of chemical compounds. A Soxhlet extractor was used to prepare the plant extract from which 18.284% extract was obtained from the dried sample of the leaves. The TPC, TFC, TAA and IC50 DPPH content in methanolic extract was found 52.22 ± 0.249 mg GAE/g DE, 258.04±2.001 mg RE/g, 166.47±0.231 mg AAE/g and 44.0419 μg/ml simultaneously. Notably, most effective antibacterial activity was reported in E. coli in which the extract displayed the inhibition zone of 15.330 ±1.530 mm. The HR-LCMS analysis of the crude extract was found to contain 117 compounds (39 unidentified and 78 identified compounds). Among them, some of these were found very potential for the medicinal aspects, i.e., Lentiginosine, Flurandrenolide, Polidocanol, Irinotecan, Glyurallin B, Hyperoside, Cynaroside, Pibutidine, Syzginin B, Capvipetin D, Ganoderic acid K, Ginsenoside-F3, Paramethansone, Medicanine etc. The specific characteristics of the molecules make them useful for the treatment of different diseases, i.e. HIV, various types of skin related diseases (skin allergies, itching, swelling etc.), removal of spider veins, typical cancers like colon and rectal, liver, lung and carnival. Besides this, these molecules also work on viral (potential SARC-COV-2 binder) and bacterial infection. Other important aspects of the molecules are: antihypertensive agent, immunomodulating, anti-inflammatory agent, vasodilator agent etc. Whereas 25 compounds are bio-logically active.Additionally, HR-LCMS inspection unveiled that the plant extract contains various classes of compounds like terpenoids, alkaloids, flavonoids, steroids and tannins. These are active metabolites and are also potential biomarkers for the exceptional biological properties. To sum up, the extraction of the molecules from the plant as in its natural form depends on the polarity of solvent used in the process. Therefore, this study recommends to extract the molecules in the solvent of different polarity and characterizes them to get all information of the biological molecules. Keywords: Phytochemical screening, Antioxidant activity, Antimicrobial Activity, DPPH, LCMS spectra analysis.