Browsing by Subject "Blood samples"
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Item Sero Prevalence of Brucellosis in Pigs in 6 VDCs of Rupandehi District, Nepal(Department of Zoology, 2014) Poudel, Shree RamThe objective of the present study is to determine the prevalence of brucellosis in pigs in 6 VDCs of Rupandehi district, Nepal. For this a cross-sectional study was conducted.Serum samples of 103 pigs were taken from 6 VDCs of Rupandehi district namely Devdaha, Dudhrakshya, Majuwa, Motipur, Parrohoa and Saljhandi. Fresh blood samples were collected from the ear vein of the farm pigs and were centrifuged to separate the serum from the blood.The separated serum samples by centrifuge were transported to NZFHRC laboratory for testing by maintaining proper cold chain condition. In the labs the test was done by qualitative slide agglutination test (SAT). Out of 103 samples sero-positive for brucellosis was found to be 13.59% (14/103).Among 53 samples of femalepigs, 15.09% were found to be sero-positive for brucellosis where as out of 50 samples of the male pigs 12% were found to be sero-positive for Brucella antibody. Group-wise 5.26% from 38 samples of 0-3 months age group, 11.90 % from 42 samples of 3-6 months age group, 21.05% from 19 samples of 6-9 months age group and 75% from 4 samples of age above 9 months were found to be sero-positive for Brucella antibody.Similarly 15.38% from 52 samples of exotic breed group, 10 % from 10 samples of local breed group and 12.19% from 41 samples of crossbreed group were found to be sero-positive for Brucella antibody. The result from the analysis seems statistically significant in terms of age wise(p=0.001) whereas it seems statistically insignificant in terms of sexwise (p=0.647)and breed wise(p=0.852).This study shows an alarming situation notonly in the Rupandehi district but also contribute in the public health sector of the country. By implementing strict and appropriate prevention and control strategy as adopted by many developed countries it can be possible to eradicate this disease from Nepal.Item Sero-Epidemiologial Study on Typhoid Fever In Kailali District, Far-Western Region of Nepal (A Hospital Based Study)(Department of Zoology, 2007) Bohara, Hari SinghA Sero-epidemiological Study on Typhoid Fever in Kailali district was conducted between June 2005andMay 2006. During the study period, 936 blood samples from the suspected patients visiting Seti Zonal Hospital from various localities of Kailali district were collected and examined for the presence of Salmonella antibody to rule-out infection of Typhoid fever.A total of 936serumsamplestestedby Widal test, the incidence of Typhoid fever was found to be 30.87%.The sex-wise incidence of Typhoid fever was found slightly more in males (31.02%)than in females (30.69%). The highest incidence of Typhoid fever was found in the age group 10-19 yrs (35.31%) while the least incidence of Typhoid fever was found in the age group 60-69 yrs (9.37%) and no case of Typhoid fever was recorded in the age group of 70 yrs and above. The incidence rate of Typhoid fever in Kailali district was found 0.46 perthous and per year. The sex wise incidence rate of Typhoid fever in Kailali district was found slightly more in male (0.51/1000 /year) than in female (0.42/1000 /year).The case fatality rate due to Typhoid fever in Kailali district wasnot found. Similarly, incidence of Typhoid fever was found to be the highest in the months of August (42.19%) and the leastin the month of January (9.37%). The questionnaire survey revealed that the predominant symptoms of Typhoid fever among 289 Typhoid patients were fever in 100% patients, intense headache in84.43%, abdomen pain in 34.95%, diarrhoea and loose stool in 40.83%, vomiting and nausea in 29.76% and cough in 28.37% patients. The questionnaire survey also revealed that most important risk factors for the transmission of Typhoid fever among community people are lack of awareness,unhygenic conditions of drinking water source, improper food consuming practice and environmental pollution etc.