Sero Prevalence of Brucellosis in Pigs in 6 VDCs of Rupandehi District, Nepal
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Department of Zoology
Abstract
The objective of the present study is to determine the prevalence of brucellosis in pigs in 6 VDCs of Rupandehi district, Nepal. For this a cross-sectional study was conducted.Serum samples of 103 pigs were taken from 6 VDCs of Rupandehi district namely Devdaha, Dudhrakshya, Majuwa, Motipur, Parrohoa and Saljhandi. Fresh blood samples were collected from the ear vein of the farm pigs and were centrifuged to separate the serum from the blood.The separated serum samples by centrifuge were transported to NZFHRC laboratory for testing by maintaining proper cold chain condition. In the labs the test was done by qualitative slide agglutination test (SAT). Out of 103 samples sero-positive for brucellosis was found to be 13.59% (14/103).Among 53 samples of femalepigs, 15.09% were found to be sero-positive for brucellosis where as out of 50 samples of the male pigs 12% were found to be sero-positive for Brucella antibody. Group-wise 5.26% from 38 samples of 0-3 months age group, 11.90 % from 42 samples of 3-6 months age group, 21.05% from 19 samples of 6-9 months age group and 75% from 4 samples of age above 9 months were found to be sero-positive for Brucella antibody.Similarly 15.38% from 52 samples of exotic breed group, 10 % from 10 samples of local breed group and 12.19% from 41 samples of crossbreed group were found to be sero-positive for Brucella antibody. The result from the analysis seems statistically significant in terms of age wise(p=0.001) whereas it seems statistically insignificant in terms of sexwise (p=0.647)and breed wise(p=0.852).This study shows an alarming situation notonly in the Rupandehi district but also contribute in the public health sector of the country. By implementing strict and appropriate prevention and control strategy as adopted by many developed countries it can be possible to eradicate this disease from Nepal.