Browsing by Subject "Sterilization"
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Item Ex Situ Conservation of Two Orchid Species Viz. Cymbidium Elegan Slindley. and Dendrobium Densiflorum Lindl. by Tissue Culture Technique(Department of Botany, 2006) Pradhan, ShreetiCymbidium elegans Lindley. and Dendrobium densiflorum Lindl. are two important orchids especially used for horticultural purposes. Both of these species are listed as rare and critically endangered (CITES, Appendix II)For the conservation and propagation of these species, the present study was conducted to determine the germination of both the species and invitro mass propagation of D. densiflorum Lindl. by using MS medium(Murashige and Skoog medium, 1962) and MS medium supplemented with different growth regulators. MS medium supplemented with BAP 1mg/l was the most effective for in vitroseed germination of C. elegans Lindley. in which germination started after 9 weeks of inoculation where as the MS basal medium was most effective for in vitro seedgermination of D. densiflorum Lindl. in which germination was startedafter 5 weeks of inoculation. Shoot tip and root tip explants obtained fromin vitrogrown seedling ofD. densiflorum Lindl. was cultured in MS basalmedium and MS supplemented with various combinations of BAP andNAA. The maximum number of healthy shoots was observed in MS +BAP 2 mg/l + NAA 0.5 mg/l medium (4.0 shoots/culture). The appropriate medium for root tip development was found tobe MS + BAP1.5 mg/l (7.25 shoots/culture). For rooting, shoot tip explants from invitromultiplied shoots of D.densiflorum Lindl. were rooted by using different concentrations of IAA, IBA and NAA. The rooting wasobserved after 10 weeks of culture of shoot tips. MS + IBA 1.5 mg/l (4.5roots/culture) was found to be more effective for maximum number and enlargement of roots. Thein vitropropagated plantlets were subjected for acclimatization.Item Study on Essential Oil of Valeriana Jatamansii Jones(Department of Botany, 2007) Pokharel, Basanta RajIn the present investigation, phytochemical screening, biological screening and the study of essential oil constituents of hairy roots produced in-vitro and in–vivo rhizomes of Valeriana jatamansii Jones. were carried out. Phytochemical screening of different samples showed the presence of fatty acids, reducing compounds, basic alkaloids, flavone glycosides,sterol and triterpenes. Biological screening carried out using brine–shrimp nauplii showed that the ethyl acetate fraction of in–vitro rhizomes as the most appropriate fraction for study of cytotoxic activities. The amount of essential oil was found the highest in the hairy roots produced in 0.5mg/lNAA ie.(0.80%) and the minimum in the rhizomes collected from pyuthan i.e (0.56%). A total of seventeen compounds present in the essential oil could be identified by gas chromatography coupled with mass spectroscopy (GC-MS) analysis followed by mass library search. Patchoulenone, 1,2–butyl octyl ester of benzene dicarboxylic acid and bis (2-ethyl hexyl) phthalate were found only in the oil obtained from hairy roots .