Browsing by Subject "Tomato plants"
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Item Nitrogen Fixation Evaluation of Azotobacter Chroococcum Isolated from Soil of Forest(Deparment of Microbiology, 2022) Tolangi, PriskilaWith the continuous use of chemical fertilizers in Agriculture system may help to produce sufficient amount of food for growing population but these chemicals have an adverse effect both on the environment and living organisms. Biofertilizers are a promising alternative to hazardous chemical fertilizers and gaining importance for attaining sustainable agriculture. They play key role in maintaining soil health and plants. Microorganisms can interact with the crop plants and enhance their immunity, growth, and development. Nitrogen, Phosphorous, Potassium are the essential nutrients required for the growth of crops, but these nutrients are naturally present in insolubilized forms. Certain microorganisms make them soluble and can be easily assimilated by plants for their growth and development. In this study, Azotobacter chroococcum were isolated from forest surroundings of Panbari Dharan. 25 soil samples were collected in sterile bag and cultured in Ashby’s media by serial dilution and incubated at 30˚C for 5 days. Morphological, cultural, biochemical characterization was done and bacteria were identified. Isolated A. chroococcum was cultured in Ashby’s broth and inoculated on tomato seedlings by root dipping technique. It was found that tomato plants inoculated with A. chroococcum has significant growth in height, no. of leaves and root length than that of untreated tomato plants. The research result showed that this strain had positive effect on the increase of nitrogen content of soil. On the base of this study, it is concluded that Azotobacter chroococcum has positive effect in growth of plants. It could be good biofertilizer as an alternative to nitrogen fertilizer. Keywords: soil, free-living nitrogen fixers, nitrogen, PGPR effects, tomato plantsItem Prevalence of Nematodes in Rhizosphere of tomato (solanum lycopersicum linnaeus, 1753) plants in to different high tunnel farms in Dahachowk, Kahmandu, Nepal(Department of Zoology, 2022) Acharya, DeepaThe present study was conducted to determine nematode prevalence in tomato plants in two monoculture tunnel farms in Dahachowk. A total of 40 soil samples were collected from November-January at the time of harvest and subjected to Baermann set up for nematode isolation and extraction. The study reported occurrence of both parasitic and free living nematodes. 9 nematode genera belonging to 3 orders were identified. Among them 56% were parasitic and 44% were free living.Among parasitic nematodes, 22.9% Meloidogyne juvenile, 20.84% Tylenchorhynchus, 12.99% Helicotylenchus, 11.88% Hoplolaimus and 0.5% Discocriconemellawere encountered while among free living, 27.19% Rhabditis, 1.91% Dorylaimus, 1.4% Eudorylaimus and 0.35%Filenchus werereported. In general, Meloidogyne juvenileandRhabditis was the most frequent parasitic and free living genus with 22.96% and 27.19% composition while DiscocriconemellaandFilenchus were the least frequent parasitic and free living genus with 0.5% and 0.3% composition in both tunnels.Also soil samples from both tunnels were found to be acidic with average pH of 4.6 and 4.1 while average moisture measured were 93.22% and 77.42% for both tunnels. No significant differences (p>0.05) were observed in nematode distribution from both tunnels in relation to depth, pH and moisture contents. However, findings suggested mixed nematode diversity that tunnel 2 to having more nematode prevalence than tunnel 1 showing phyto-pathogenic tendencies for hybrid mono-culture practice. Therefore, regular researches on particular criterion can unfold hidden secrets on nematode prevalence for the region.