Prevalence of Nematodes in Rhizosphere of tomato (solanum lycopersicum linnaeus, 1753) plants in to different high tunnel farms in Dahachowk, Kahmandu, Nepal
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Department of Zoology
Abstract
The present study was conducted to determine nematode prevalence in tomato plants in two
monoculture tunnel farms in Dahachowk. A total of 40 soil samples were collected from
November-January at the time of harvest and subjected to Baermann set up for nematode
isolation and extraction. The study reported occurrence of both parasitic and free living
nematodes. 9 nematode genera belonging to 3 orders were identified. Among them 56% were
parasitic and 44% were free living.Among parasitic nematodes, 22.9% Meloidogyne juvenile,
20.84% Tylenchorhynchus, 12.99% Helicotylenchus, 11.88% Hoplolaimus and 0.5%
Discocriconemellawere encountered while among free living, 27.19% Rhabditis, 1.91%
Dorylaimus, 1.4% Eudorylaimus and 0.35%Filenchus werereported. In general, Meloidogyne
juvenileandRhabditis was the most frequent parasitic and free living genus with 22.96% and
27.19% composition while DiscocriconemellaandFilenchus were the least frequent parasitic
and free living genus with 0.5% and 0.3% composition in both tunnels.Also soil samples
from both tunnels were found to be acidic with average pH of 4.6 and 4.1 while average
moisture measured were 93.22% and 77.42% for both tunnels. No significant differences
(p>0.05) were observed in nematode distribution from both tunnels in relation to depth, pH
and moisture contents. However, findings suggested mixed nematode diversity that tunnel 2
to having more nematode prevalence than tunnel 1 showing phyto-pathogenic tendencies for
hybrid mono-culture practice. Therefore, regular researches on particular criterion can unfold
hidden secrets on nematode prevalence for the region.