Detection of Inducible Clindamycin Resistance Along With E-Test in Staphylococcus aureus Isolated from Clinical samples

Abstract

Staphylococcus aureus (S.aureus) is commonly isolated pathogen from clinical specimen with increasing trend of antimicrobial resistance. The main aim of this study was to isolate Staphylococcus aureus from different clinical sample and tode termine their susceptibility patterns. This study was conducted from August2017 to January 2018. During this time period, a total of 3893 different clinical samples were processed by standard microbiological techniques. Following identification, isolates were subjected to antibiotic susceptibility testing using modified Kirby-Bauer disc diffusion method and further admitted for screening of methicillin- resistant Staphylococcus aureus(MRSA), inducible clindamycin resistant Staphylococcus aure usand MIC performed by E-test. Among 3893 different clinical isolates processed in this study, S.aureus was isolated from 97 samples (7.60%) which constituted 62 (63.9%) from male and 35(36.1%) from female; 60 (61.9%) from inpatient and 37 (38.1%) from outpatient.59 (60.8%) isolates were obtained from MRSA. In this study, all the MRSA isolates were obtained multidrug resistant (MDR), whereas 71.05% that meansout of 27 of 38 of MSSA isolates were MDR. Although none of the isolates shows showed constitutive resistance to clindamycin, there were 35 inducible clindamycin resistant isolates; 12 from MSSA and 23 from MRSA and theisolates were found susceptible to vancomycin by E Test method. On conclusion,observation of D- effect among some isolates provokes the necessities for development of new strategies. Keywords:Staphylococcus aureus, MRSA, Inducible clindamycin resistance, E test