Correlation of Multidrug Resistant and Plasmid Profile in ESBL Producing Escherichiacoli In UTI Patients

Abstract

Urinary Tract Infection (UTI) is one of the most common infectious disease ranking next to upper respiratory tract infection, is the cause of morbidity and mortality in human. Therefore, studying bacterial pathogens causing UTI and their drug susceptibility patterns is of the highest priority. Infections caused by Escherichia coli have become a significant worldwide public health problem.Furthermore, the situation is worsening due to advent of increased antibiotic resistance due to the evolution of multi-resistant antibiotic plasmid genes.Extended Spectrum Beta lactmases (ESBLs) are plasmid mediated and the seen zyme producing organisms exhibit co resistance to many other classes of antibiotics. A total of 1258 urine samples were collected from May2013 to November 2013.Bacterial isolates were identified using standard biochemical tests and antimicrobial susceptibility pattern was determined using the Kirby–Bauer’s disk diffusion method following CLSI guidelines. Confirmation of the extended spectrum β-lactamase (ESBL) E.coli was done as guided by CLSI guidelines.Plasmid DNA isolation of all the ESBL positive strains was done by alkalinelysis method. Inthe present study, out of 303isolates, 198isolates were ofE.coli. These isolates were tested for antibiotic sensitivity, MDR, ESBL and plasmid profiles. The results revealed that more than 50% of the isolates exhibited multi-drug resistance. Out of 76ESBL E.coli isolates, 41(53.9%) were found to possess plasmids. Some isolates possess single plasmid while other had multiple plasmids with different size ranged from 1 kb to 10kb.The studies show good prospects for further research in the same area to explore and assign definite cause for antibiotic resistance, multi drug resistance and ESBL production. Keywords: Prevalence; Urinary tract infection; Antimicrobial susceptibility pattern;MDR;ESBL; Plasmid Profile.