Medically Important Vibrios in the Sewage of Kathmandu Vally During Winter Season

Abstract

The natural lodgment of Vibrios in aquatic environment is the potential source for possible Vibrios infection. The study was aimed to isolate medically important Vibrios from the sewage of Kathmandu (KTM) Valley during winter season. A total of 60 random samples were taken from the different sewerages sites discharging sewage into the Bagmati River between 10th November 2008 to 6th February 2009 employing principle based on Moore’s Technique. The samples [Moore’s technique based swabs were submersed in alkaline peptone water (broth) pH of 8.6] were transported to Research Laboratory of Central Department of Microbiology in cold condition. The samples were incubated at 370C for 8 hours enrichment followed by culture on thiosulfate-citrate-bile salts-sucrose (TCBS) agar and incubated at 370C for overnight. The TCBS plates showing Vibrios like colonies were subjected for biochemical identification. V. cholerae suspected isolates were subjected to sero-typing using polyvalent V. cholerae 01 anti-sera. In this study, from 60 samples, 57 medically important Vibrios were isolated of which V. parahemolyticus (45.6%) was dominating isolates followed by V. cholerae (21%) and others. On sero-typing, all V. cholerae isolates were V. cholerae Non-O1 (NAG). NAG (n=12) isolates were tested for antibiotic susceptibility test (AST) using disc diffusion technique. Of total AST isolates, two isolates were found to be multidrug resistant. Since, Sewage of KTM Valley harbor the medically important Vibrios which could be the potential source for outbreak and demands proper sanistation and awareness among Valley residents.