Antifungal activities of some medicinal plant extracts and Trichoderma spp. against stemphylium vesicarium of allium sativum

Abstract

The extracts of eight aromatic plants viz. Cuscuta reflexa, Syzygium aromaticum, Allium cepa, Cinnamomum zeylanicum, Solanum xanthocarpum, Phyllanthus emblica, Cinnamomum camphora and Equisetum diffusum were assessed in-vitro for antifungal activity against Stemphylium vesicarium; the causal organism of leaf blight of garlic. Pathogenicity test was confirmed by inoculating the pathogen into healthy garlic plant. The assessment for fungitoxicity was carried out by poisoned food technique using five different concentrations (20%, 40%, 60%, 80% & 100%) against the test fungus in terms of percentage of mycelial growth inhibition. Among the test plants, the extracts of Cuscuta reflexa, Syzygium aromaticum and Allium cepa were able to inhibit the mycelial growth completely. Trichoderma spp. was tested to determine its effect on mycelia growth of S. vesicarium on PDA medium. The mycelia growth was totally inhibited by Trichoderma spp. showing its fungitoxic properties.