Histological Study on Effect of Agave Americana on Stomach and Intestine of Clarias Batrachus.

Abstract

The study was carried on freshwater fish Clarias batrachus which was subjected to 24hours, 48hours, 72hours and 96hours administration of Agave americana of sub- lethal concentration. Research was conducted to observe the histopathological changes in the stomach and intestine. Fish meal compromised liver of chicken and soyabean. After sacrificing the fish 0.5cm х 0.5cm) from its stomach and intestine were fixed by immersion in Bouin’s solution. The specimen were processed through routine paraffin embedding technique and cut at 7µm and stained with hematoxylin and eosin. Histopathological changes which includes ; Necrosis, rupture of serosa layer, disorganization of villi, enlargements of glands, hemorrhage and congestion in the sub mucous layer in intestine and necrosis of columnar epithelial cells, accumulation of toxicity within the tissue spaces, atrophy and shrinkage of mucosa, enlargement of glands in stomach of test species. In 96 hours of treatment all the treated fishes were died.