Nanoparticle integrated microbial water splitting for carbonate reduction in co2 capturing

Abstract

The global population is increasing day by day and the problems generated by them also increasing in the same proportion. The global warming mainly due to the anthropogenic activities is among them and innovative approaches are required to tame this. Among the agents involved in global warming are nitrous oxide (N2O), carbon dioxide (CO) but N2O has around 300 times the heat-trapping capacity than CO and could be released in atmosphere through nitrogen cycling of nitrogenous chemical fertilizers. Moreover, having heterotrophic bacterial bio-fertilizer supplement that has ability to utilize ammonia and nitrate could be an alternative to prevent N22O release. Thus, bio-fertilizer could be alternative to counter the negative impact of indiscriminate use of chemical fertilizers. Hence, one of the bio-fertilizers, Azospirillum species, was isolated and characterized through Gram’s staining and biochemical tests. Then ammonium ions present in nitrogen free broth (Nfb) culture media were quantified to select the best strain for development as bio-fertilizer. Similarly, syntrophic growth ability of isolates in modified nitrogen and carbon free (NCF) media indicated that the isolates can reduce atmospheric CO. Then, protocol was optimized for the development of cellulose producing strain directly from reduce carbon source obtain from CO 2reduction byisolates. Water splitting mechanism also done by using MFCs where the bacterial culture was incubated. Genomic DNA extracted from the DH5α E.coli and bcsA and adrA gene were amplified by using designed primers. Cellulose genetic engineering was performed using these genes upon digestion and ligation of bcsA gene and adrA gene in pET28a+ vector. Transformation was successfully done in E.coli DH5α. Finally transforming was validated through single restriction digestion of extracted plasmids. Thus, it is suggested that engineering of the isolate for higher COreduction and cellulose production could support micro cellulose production. 2 Keywords: Bio-fertilizer, Azospirillum, Syntrophic growth, COreduction, water splitting mechanism, Cellulose genetic engineering