Culture and Characterization of Stem cells Isolated from Bovine Umbilical Card Blood and study the effect of Clemati's Buchananiana DC Extract

Abstract

Stem cells are undifferentiated cells that can perpetually divide and differentiate into different types of cells. These cells possess high potential for regenerative therapy. Umbilical cord blood (UCB) is residual blood contained in the umbilical cord discarded after the birth of the baby. It is rich in hematopoietic stem cells and different types of other mesenchymal stem cells. Sample was collected from bovine umbilical cord and cultured in DMEM low glucose medium. Two different types of cells were isolated, among them the floating cells were subcultured for up to 9 passages. Till the 9 th passage very active growth of cells was observed. These dividing cells on wright staining microscopy show different types of cell population morphologically similar to blood cells i.e., lymphocytes, erythrocytes, granulocytes, monocytes. Presence of these cells till 8 th , 9 th

passage indicate there is the source of these blood cells i.e., hematopoietic stem cells. These cells were treated with the phytochemical extracted from the ethnomedicinal plant Clematis buchananiana. On treatment with phytochemicals, cell proliferation was observed. Keywords: Umbilical cord blood, stem cell, Clematis buchananiana, Alkaloids, Phenolics, wright stain, MTT assay, Phytochemicals, PBMC, Regenerative Therapy, Proliferation